Functional analysis of articular cartilage deformation, recovery, and fluid flow following dynamic exercise in vivo
Functional analysis of articular cartilage deformation, recovery, and fluid flow following dynamic exercise in vivo
复制标题
DOI：
10.1007/s004290050291
复制
发表时间：
1999-10-01
期刊：
影响因子：
--
通讯作者：
Reiser, M
中科院分区：
文献类型：
--
作者：
Eckstein, F;Tieschky, M;Reiser, M
关节软骨的功能取决于组织基质和与蛋白多糖分子结合的间质液之间的相互作用。机械负荷已被证明参与软骨细胞的代谢调节和基质变性。因此，本研究的目的是分析人体关节软骨在体内动态加载后的变形、恢复和流体流动。7名志愿者的髌骨在身体休息和屈膝后进行成像，具有专门优化的脂肪抑制FLASH-3D磁共振（MR）序列。为了测量软骨变形，采用3D数字图像分析确定髌骨软骨的总体积。膝关节屈曲50次后髌骨软骨变形率为2.4%~ 8.6%，屈曲100次后髌骨软骨变形率为2.4%~ 8.5%。以15分钟的间隔重复进行动态运动不会引起进一步的变形。膝关节弯曲100次后，软骨需要90 min以上才能从负荷中恢复。松弛期间的液体流速范围为1.1 - 3.5 mm（3）/min（每平方厘米关节面0.08 - 0.22 mm（3）/min），与膝关节弯曲后的个体变形程度高度相关。这些数据提供了关节软骨恢复的第一个量化和在体内完整关节中软骨基质和周围组织之间的流体流动速率。在生活中的测量打开了可能性的机械软骨特性的个体间的变化，发展中的关节衰竭的易感性，以评估负载转移过程中的流体相和固体软骨基质之间的负载分配，并确定机械诱导的流体流动的作用，在软骨细胞的代谢活性的调节。
The function of articular cartilage depends on the interaction between the tissue matrix and the interstitial fluid bound to the proteoglycan molecules. Mechanical loading has been shown to be involved in both the metabolic regulation of chondrocytes and in matrix degeneration. The purpose of the present study was therefore to analyze the deformation, recovery, and fluid flow in human articular cartilage after dynamic loading in vivo. The patellae of 7 volunteers were imaged at physical rest and after performing knee bends, with a specifically optimized fat-suppressed FLASH-3D magnetic resonance (MR) sequence. To measure cartilage deformation, the total volume of the patellar cartilage was determined, employing 3D digital image analysis. Patellar cartilage deformation ranged from 2.4 to 8.6% after 50 knee bends, and from 2.4% to 8.5% after 100 knee bends. Repeated sets of dynamic exercise at intervals of 15 min did not cause further deformation. After 100 knee bends, the cartilage required more than 90 min to recover from loading. The rate of fluid flow during relaxation ranged from 1.1 to 3.5 mm(3)/min (0.08 to 0.22 mm(3)/min per square centimeter of the articular surface) and was highly correlated with the individual degree of deformation after knee bends. The data provide the first quantification of articular cartilage recovery and of the rate of fluid flow between the cartilage matrix and surrounding tissue in intact joints in vivo. Measurement in the living opens the possibility of relating interindividual variations of mechanical cartilage properties to the susceptibility of developing joint failure, to assess the load-partitioning between the fluid phase and solid cartilage matrix during load transfer, and to determine the role of mechanically induced fluid flow in the regulation of the metabolic activity of chondrocytes.