tRNA Stabilization by Modified Nucleotides
tRNA Stabilization by Modified Nucleotides
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DOI：
10.1021/bi100408z
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发表时间：
2010-06-22
期刊：
影响因子：
2.9
通讯作者：
Helm, Mark
中科院分区：
文献类型：
--
作者：
Motorin, Yuri;Helm, Mark
转录后核糖核苷酸修饰是tRNA中研究得最好的一种现象，它发生得最频繁，化学多样性也很大。本文综述了tRNA结构核心中固有的修饰网络，这些修饰网络控制着结构的灵活性和刚性，从而微调分子达到峰值性能并调节其稳态水平。RNA修饰的结构效应范围从纳米级的重排到埃级构象空间的细微限制。由核苷酸修饰引起的结构稳定性增加了热稳定性，并转化为对碱基和核酸酶非特异性降解的保护。最近才发现的几种低修饰tRNA特异性降解机制，提供了结构和代谢稳定性之间的联系。
Post-transcriptional ribonucleotide modification is a phenomenon best studied in tRNA, where it occurs most frequently and in great chemical diversity. This paper reviews the intrinsic network of modifications in the structural core of the tRNA, which governs structural flexibility and rigidity to fine-tune the molecule to peak performance and to regulate its steady-state level. Structural effects of RNA modifications range from nanometer-scale rearrangements to subtle restrictions of conformational space on the angstrom scale. Structural stabilization resulting from nucleotide modification results in increased thermal stability and translates into protection against unspecific degradation by bases and nucleases. Several mechanisms of specific degradation of hypomodified tRNA, which were only recently discovered, provide a link between structural and metabolic stability.