Signaling related with biphasic effects of bisphenol A (BPA) on Sertoli cell proliferation: A comparative proteomic analysis
Signaling related with biphasic effects of bisphenol A (BPA) on Sertoli cell proliferation: A comparative proteomic analysis
复制标题
与双酚 A (BPA) 对支持细胞增殖的双相效应相关的信号转导：比较蛋白质组学分析
DOI：
10.1016/j.bbagen.2014.05.018
复制
发表时间：
2014-09-01
影响因子：
3
通讯作者：
Wang, Hong-Sheng
中科院分区：
文献类型：
--
作者：
Ge, Li-Chen;Chen, Zhuo-Jia;Wang, Hong-Sheng
Background: Biphasic effects on cell proliferation of bisphenol A (BPA) can occur at lesser or greater exposures. Sertoli cells play a pivotal role in supporting proliferation and differentiation of germ cells. The mechanisms responsible for inverse effects of great and low concentrations of BPA on Sertoli cell proliferation need further study.Methods: We utilized proteomic study to indentify the protein expression changes of Sertoli TM4 cells treated with 10(-8) M and 10(-5) M BPA. The further mechanisms related to mitochondria, energy metabolism and oxidative stress were investigated by ciRT-PCR and Western-blotting analysis.Results: Proteomic studies identified 36 proteins and two major clusters of proteins including energy metabolism and oxidative stress expressed with opposite changes in Sertoli cells treated with 10(-8) M and 10(-5) M BPA, respectively, for 24 h. Exposure to 10(-5) M BPA resulted in greater oxidative stress and then inhibited cell proliferation, while ROS scavenger NAC effectively blocked these effects. Exposure to 10(-8) M BPA caused higher intercellular ATP, greater activities of mitochondria, and resulted in significant proliferation of TM4 cells, while oligomycin A, an inhibitor of ATP synthase, abolished these growth advantages.Conclusions: Our study demonstrated that micromolar BPA inhibits proliferation of Sertoli cells by elevating oxidative stress while nanomolar BPA stimulates proliferation by promoting energy metabolism.General significance: Micromolar BPA inhibits cell proliferation by elevating oxidative stress while nanomolar BPA stimulates cell proliferation by promoting energy metabolism. (C) 2014 Elsevier B.V. All rights reserved.
