Structural analysis of interspersed repetitive polymerase III transcription units in human DNA.
Structural analysis of interspersed repetitive polymerase III transcription units in human DNA.
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DOI：
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发表时间：
1981-03
影响因子：
14.9
通讯作者：
J. Pan;James T. Elder;C. Duncan;S. Weissman
中科院分区：
文献类型：
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作者：
J. Pan;James T. Elder;C. Duncan;S. Weissman
体外用作RNA聚合酶III模板的两个克隆的人DNA片段的核苷酸序列证实了它们作为人散布重复DNA序列的Alu家族成员的身份（1，2）。这些序列在基因组中的散布和重复性质通过来自这些克隆之一的切口翻译的DNA与总基因组DNA和来自基于λ Ch4A的人类基因组文库的单个随机克隆的DNA的杂交来证明。非保守序列的短的、直接的末端重复位于300个核苷酸的Alu家族保守序列的侧翼。在Alu家族序列中发现了一个40个核苷酸的区域，该区域在下游135个核苷酸处直接重复。该40个核苷酸的序列在鼠B1散布重复序列家族中发现过一次（8）。这一点和其他证据表明，人类Alu家族类似于鼠B1序列的部分重复。
The nucleotide sequences of two cloned fragments of human DNA which function as templates for RNA polymerase III in vitro confirm their identities as members of the Alu family of human interspersed repetitive DNA sequences (1,2). The interspersed and repetitive nature of these sequences in the genome was demonstrated by hybridization of nick-translated DNA from one of these clones to total genomic DNA and to DNA of individual random clones from a lambda Ch4A-based human genomic library. Short, direct terminal repeats of non-conserved sequence flank the 300 nucleotide Alu family conserved sequence. Within the Alu family sequence is found a 40-nucleotide region which is directly repeated 135 nucleotides downstream. This 40 nucleotide sequence is found once in the murine B1 interspersed repetitive sequence family (8). This and other evidence indicates that the human Alu family resembles a partial duplication of the murine B1 sequence.