Self-decorating cells via surface-initiated enzymatic controlled radical polymerization.
Self-decorating cells via surface-initiated enzymatic controlled radical polymerization.
复制标题
通过表面引发的酶控制自由基聚合来自我装饰细胞。
DOI：
10.1039/d3nr04008a
复制
发表时间：
2023
期刊：
影响因子：
6.7
通讯作者：
Belluati A
中科院分区：
文献类型：
--
作者：
Belluati A
通过创新地使用表面展示的辣根过氧化物酶，这项工作探索了bioRAFT聚合和bioATRP的酶催化，以促进酿酒酵母细胞表面的聚合物合成，bioATRP优于bioRAFT聚合。用合成聚合物对活酵母细胞进行所得表面改性允许酵母表型的显著变化，包括生长概况、聚集特征以及非天然酶与细胞表面上的可点击聚合物的缀合，从而在生物正交细胞表面工程中开辟了新途径。
Through the innovative use of surface-displayed horseradish peroxidase, this work explores the enzymatic catalysis of both bioRAFT polymerization and bioATRP to prompt polymer synthesis on the surface of Saccharomyces cerevisiae cells, with bioATRP outperforming bioRAFT polymerization. The resulting surface modification of living yeast cells with synthetic polymers allows for a significant change in yeast phenotype, including growth profile, aggregation characteristics, and conjugation of non-native enzymes to the clickable polymers on the cell surface, opening new avenues in bioorthogonal cell-surface engineering.