Electrochemical Aptamer-Based Sensors: A Platform Approach to High-Frequency Molecular Monitoring In Situ in the Living Body.
Electrochemical Aptamer-Based Sensors: A Platform Approach to High-Frequency Molecular Monitoring In Situ in the Living Body.
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DOI：
10.1007/978-1-0716-1803-5_25
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发表时间：
2022-01-01
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影响因子：
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通讯作者：
Plaxco, Kevin W
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文献类型：
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作者：
Dauphin-Ducharme, Philippe;Ploense, Kyle L;Plaxco, Kevin W
历史上，对活体中特定分子的监测需要去除样本（例如抽血、微透析），然后通过繁琐的实验室流程进行分析。这一规则的少数例外（例如，葡萄糖、丙酮酸、单胺）是使用依赖于其目标的特定酶促或氧化还原反应性的“一次性”技术来监测的，因此不能推广到其他目标的测量。为此，我们开发了基于电化学适体 (E-AB) 的体内传感器，这是一种基于受体的模块化测量技术，独立于其目标的化学反应性，因此有可能推广到广泛的分析物。为了进一步让其他研究人员采用这种体内分子测量方法并加速其最终转化为临床，我们在此介绍我们用于制造和使用静脉 E-AB 传感器的标准协议。
The monitoring of specific molecules in the living body has historically required sample removal (e.g., blood draws, microdialysis) followed by analysis via cumbersome, laboratory-bound processes. Those few exceptions to this rule (e.g., glucose, pyruvate, the monoamines) are monitored using "one-off" technologies reliant on the specific enzymatic or redox reactivity of their targets, and thus not generalizable to the measurement of other targets. In response we have developed in vivo electrochemical aptamer-based (E-AB) sensors, a modular, receptor-based measurement technology that is independent of the chemical reactivity of its targets, and thus has the potential to be generalizable to a wide range of analytes. To further the adoption of this in vivo molecular measurement approach by other researchers and to accelerate its ultimate translation to the clinic, we present here our standard protocols for the fabrication and use of intravenous E-AB sensors.