Sodium bisulfite enhances photosynthesis in rice by inducing Rubisco activase gene expression
Sodium bisulfite enhances photosynthesis in rice by inducing Rubisco activase gene expression
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DOI：
10.1007/s11099-014-0044-7
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发表时间：
2014-09-01
期刊：
影响因子：
2.7
通讯作者：
Jiang, D. -A.
中科院分区：
文献类型：
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作者：
Chen, Y.;Jin, J. -H.;Jiang, D. -A.
为了研究亚硫酸氢盐对光合作用的促进作用，以水稻为材料，进行了盆栽试验。测定水稻叶片喷施NaHSO3后的Rubisco活性和含量，以及Rubisco激活酶(RCA)基因的表达。NaHSO3处理显著提高了叶片的净光合速率(P(N))、羧化效率、最大羧化速率、核酮糖-1，5-二磷酸再生速率、初始Rubisco活性以及RCA蛋白和mRNA浓度。因此，NaHSO3对P(N)的增强作用可能直接归因于在转录和翻译水平上诱导RCA基因的表达。因此，RCA的增加在体内调节了Rubisco的初始活性。
To investigate how bisulfite promotes photosynthesis, a pot experiment was conducted with rice (Oryza sativa L.) plants to determine Rubisco activity and content, and Rubisco activase (RCA) gene expression after spraying NaHSO3 on rice leaves. The NaHSO3 treatment promoted significantly net photosynthetic rate (P (N)), carboxylation efficiency, maximum carboxylation rate, ribulose-1,5-bisphosphate regeneration rate, initial Rubisco activity, and RCA protein and mRNA concentrations. Therefore, the NaHSO3 enhancement of P (N) could be directly attributed to induction of RCA gene expression both at the transcription and translation levels. Thus, the increased RCA regulated the initial Rubisco activity in vivo.