Fos and Egr1 expression in the rat brain in response to olfactory cue after taste-potentiated odor aversion retrieval
Fos and Egr1 expression in the rat brain in response to olfactory cue after taste-potentiated odor aversion retrieval
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DOI：
10.1101/lm.148706
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发表时间：
2006-03-01
影响因子：
2
通讯作者：
Cattarelli, M
中科院分区：
文献类型：
--
作者：
Dardou, D;Datiche, F;Cattarelli, M
当一种气味与一种迟发性疾病相结合时，大鼠会获得相对较弱的气味厌恶。相反，如果嗅觉提示与味觉提示同时出现，大鼠会对嗅觉提示产生强烈的厌恶，并伴有延迟性疾病。这种调节作用被称为味觉增强气味厌恶学习（TPOA）。TPOA是一个研究神经可塑性机制的有趣模型，因为它的鲁棒性和快速获取。然而，TPOA检索中涉及的神经基质尚未得到很好的表征。为了解决这个问题，我们使用免疫细胞化学检测诱导转录因子编码的立即早期基因Fos和Egr 1。将口渴的雄性大鼠条件化为TPOA学习，并在3 d后在学习气味的存在下进行检索。与所有对照组相比，厌恶组大鼠基底外侧杏仁核、岛叶皮质和海马中Fos和Egr 1的表达均显著增加。神经元活动的模式似乎不太可能与唯一的氯化锂注射。Fos和Egr 1在内嗅皮层和杏仁中央核的表达模式相反，提示这两个标记物在TPOA的提取中有不同的作用。
When an odor is paired with a delayed illness, rats acquire a relatively weak odor aversion. In contrast, rats develop a strong aversion to an olfactory Cue paired with delayed illness if it is presented simultaneously with a gustatory cue. Such a conditioning effect has been referred to as taste-potentiated odor aversion learning (TPOA). TPOA is an interesting model for Studying neural mechanisms of plasticity because of its robustness and rapid acquisition. However, the neural substrate involved in TPOA retrieval has not been well characterized. To address this question, we used immunocytochemical detection of inducible transcription factors encoded by the immediate-early genes Fos and Egr1. Thirsty male rats were conditioned to TPOA learning, and they were submitted to retrieval in the presence of the learned odor 3 d later. Significant increases in both Fos and Egr1 expressions were observed in basolateral amygdala, insular cortex, and hippocampus in aversive rats in comparison with the all the control groups. The pattern of neuronal activity seemed unlikely to be related to the sole LiCl injection. Lastly, opposite patterns of Fos and Egr1 were noted in the entorhinal cortex and the central nucleus of amygdala, Suggesting a differential involvement of these markers in retrieval of TPOA.