Uncoating of HIV-1 requires cellular activation
Uncoating of HIV-1 requires cellular activation
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DOI：
10.1016/j.virol.2005.02.028
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发表时间：
2005-06-20
期刊：
影响因子：
3.7
通讯作者：
Puthavathana, P
中科院分区：
文献类型：
--
作者：
Auewarakul, P;Wacharapornin, P;Puthavathana, P
脱壳是病毒复制周期中必不可少的一步。关于HIV脱壳的机理和要求，人们知之甚少。利用体外去包被模型，我们证明了活化的CD4+淋巴细胞的裂解物能有效地诱导HIV-1的去包被，而静止的CD4+淋巴细胞裂解液不能去包被HIV-1核心。脱壳活性与诱导病毒基因组的体外逆转录有关。使用细胞周期中被阻断的CD4+淋巴细胞，我们发现去包被的活性需要细胞从G(0)/G(Ta)过渡到G(1b)阶段。这些结果强烈地表明，HIV-1脱壳需要依赖于细胞周期的特定因子。通过凝胶过滤层析，可以从细胞裂解液中分离出可能的HIV-1去包被因子。(C)2005 Elsevier Inc.保留所有权利。
Uncoating is an essential step in viral replication cycle. Little is known about the mechanism and requirement of HIV uncoating. Using an in vitro uncoating model, we demonstrate here that the uncoating of HIV-1 was efficiently induced by lysate from activated CD4+ lymphocytes, while quiescent CD4+ lymphocyte lysate was unable to uncoat HIV-1 core. The Uncoating activity was associated with an induction of ill vitro reverse transcription of the viral genome. Using CD4+ lymphocytes that were arrested in cell cycle, we showed that the uncoating activity required transition of cells from G(0)/G(ta) into G(1b) stage. These results strongly suggested a requirement of cell cycle-dependent specific factors for HIV-1 uncoating. The putative HIV-1 uncoating factors could be fractionated from cell lysate by gel filtration chromatography. (c) 2005 Elsevier Inc. All rights reserved.