Viscoelastic properties of a mixed culture biofilm from rheometer creep analysis
Viscoelastic properties of a mixed culture biofilm from rheometer creep analysis
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DOI：
10.1080/0892701031000152470
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发表时间：
2003-10-01
期刊：
影响因子：
2.7
通讯作者：
Stoodley, P
中科院分区：
文献类型：
--
作者：
Towler, BW;Rupp, CJ;Stoodley, P
使用AR1000旋转盘流变仪通过蠕变分析测定混合培养物生物膜的机械性质。将生物膜直接生长在流变仪盘上，流变仪盘在恒化器中旋转12 d。所得的生物膜是异质的，厚度范围从35 μ m到50 μ m。蠕变曲线在性质上都是粘弹性的。在0.1和0.5 Pa下测试的样品的应力和应变比之间的密切协议表明，生物膜在线性粘弹性范围内进行测试，并支持使用线性粘弹性理论的本构关系的发展。实验数据拟合为4元件Burger弹簧和阻尼器模型。剪切模量（G）为0.2至24 Pa，粘性系数（η）为10至3000 Pa。这些值与先前从流动池中生物膜的流体剪切变形估计的值在相同的范围内。粘弹性生物膜模型将有助于预测剪切相关的生物膜。现象，如升高的压降、分离和生物膜在固体表面上的流动。
The mechanical properties of mixed culture biofilms were determined by creep analysis using an AR1000 rotating disk rheometer. The biofilms were grown directly on the rheometer disks which were rotated in a chemostat for 12 d. The resulting biofilms were heterogeneous and ranged from 35 mum to 50 mum in thickness. The creep curves were all viscoelastic in nature. The close agreement between stress and strain ratio of a sample tested at 0.1 and 0.5 Pa suggested that the biofilms were tested in the linear viscoelastic range and supported the use of linear viscoelastic theory in the development of a constitutive law. The experimental data was fit to a 4-element Burger spring and dashpot model. The shear modulus (G) ranged from 0.2 to 24 Pa and the viscous coefficient (eta) from 10 to 3000 Pa. These values were in the same range as those previously estimated from fluid shear deformation of biofilms in flow cells. A viscoelastic biofilm model will help to predict shear related biofilm. phenomena such as elevated pressure drop, detachment, and the flow of biofilms over solid surfaces.