Persistent Angiogenesis in the Autism Brain: An Immunocytochemical Study of Postmortem Cortex, Brainstem and Cerebellum
Persistent Angiogenesis in the Autism Brain: An Immunocytochemical Study of Postmortem Cortex, Brainstem and Cerebellum
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DOI：
10.1007/s10803-015-2672-6
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发表时间：
2016-04-01
影响因子：
3.9
通讯作者：
Whitaker-Azmitia, P. M.
中科院分区：
文献类型：
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作者：
Azmitia, E. C.;Saccomano, Z. T.;Whitaker-Azmitia, P. M.
在目前的工作中，我们进行了免疫细胞化学搜索标记正在进行的神经发生（如巢蛋白）在听觉皮层从自闭症谱系障碍（ASD）和年龄匹配的控制捐助者的尸检部分。我们在血管系统的细胞中发现了巢蛋白标记，表明血管具有可塑性。在ASD患者的死后大脑中，在上级颞叶皮质（初级听觉皮质）、梭状皮质（面部识别中心）、脑桥/中脑和小脑中观察到血管生成的证据，但对照组大脑中没有。我们发现巢蛋白和CD 34的显着增加，这是局部周细胞和内皮细胞的血管生成的标志物，分别。该标记谱指示分裂（插入）而不是发芽，血管生成指示血管处于恒定流动而不是持续扩张。
In the current work, we conducted an immunocytochemical search for markers of ongoing neurogenesis (e.g. nestin) in auditory cortex from postmortem sections of autism spectrum disorder (ASD) and age-matched control donors. We found nestin labeling in cells of the vascular system, indicating blood vessels plasticity. Evidence of angiogenesis was seen throughout superior temporal cortex (primary auditory cortex), fusiform cortex (face recognition center), pons/midbrain and cerebellum in postmortem brains from ASD patients but not control brains. We found significant increases in both nestin and CD34, which are markers of angiogenesis localized to pericyte cells and endothelial cells, respectively. This labeling profile is indicative of splitting (intussusceptive), rather than sprouting, angiogenesis indicating the blood vessels are in constant flux rather than continually expanding.