Targeted Imaging Modality Selection for Bacterial Biofilms in Chronic Rhinosinusitis
Targeted Imaging Modality Selection for Bacterial Biofilms in Chronic Rhinosinusitis
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DOI：
10.1002/lary.20705
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发表时间：
2010-02-01
期刊：
影响因子：
2.6
通讯作者：
Wormald, Peter-John
中科院分区：
文献类型：
--
作者：
Foreman, Andrew;Singhal, Deepti;Wormald, Peter-John
目的/假设：生物膜在慢性鼻-鼻窦炎(CRS)中的病因学作用日益被公认。目前对CRS生物膜的研究依赖于显微成像技术，但没有一种技术被普遍接受。本研究比较了活体/死体BacLight(Invitgen Corp.，CarlsbadCA)染色和荧光原位杂交(FISH)两种方法对CRS患者生物膜的检测和表征。研究设计：前瞻性研究。方法：20例CRS患者接受鼻内窥镜手术。结果：结合两种方法的结果，18/20(90%)的患者至少有一种方法可以显示出细菌生物膜。结合这两种技术的高生物被膜检测率表明，CRS中生物被膜的流行率可能比之前报道的更高。这些方案在15/20名患者中获得了相同的结果。根据在其余5名患者中观察到的差异，我们可以强调每种技术最合适的使用。结论：BacLight/CSLM和FISH/CSLM是生物被膜检测和表征的互补技术。这两项议定书都适用于不同的研究领域，所使用的技术的选择应基于研究议定书的具体目标。这样，我们就可以利用每种技术的优势来促进有效的研究。
Objectives/Hypothesis: Biofilms are increasingly recognized as having an etiological role in chronic rhinosinusitis (CRS). Research into biofilms in CRS currently relies on microscopic imaging techniques, none of which are universally accepted. This study compares LIVE/DEAD BacLight (Invitrogen Corp., Carlsbad, CA) staining and fluorescence in situ hybridization (FISH), both utilizing the confocal scanning laser microscope (CSLM) for biofilm determination and characterization in CRS patients.Study Design: Prospective study.Methods: Twenty CRS patients undergoing endoscopic sinus surgery were recruited for the study. Sinus mucosal tissue harvested at the time of surgery underwent both the BacLight/CSLM and FISH/CSLM protocols for biofilm determination and characterization.Results: Combining the results of both protocols, 18/20 (90%) patients had bacterial biofilms demonstrable on at least one modality. The high biofilm detection rate combining the two techniques suggests the prevalence of biofilms in CRS may be greater than previously reported. The protocols had equivalent results in 15/20 patients. Using the differences observed in the remaining five patients, we can highlight the most appropriate use for each technique.Conclusions: BacLight/CSLM and FISH/CSLM are complementary techniques for biofilm determination and characterization. Both protocols are suited to different research areas and the selection of technique used should be based on the specific objectives of the research protocol. In this way we can utilize the advantages of each technique to facilitate effective research.