Reactions of cytochrome c oxidase with sodium dithionite.
Reactions of cytochrome c oxidase with sodium dithionite.
复制标题
细胞色素c氧化酶与连二亚硫酸钠的反应。
DOI：
10.1042/bj2090175
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
P. Sarti
中科院分区：
文献类型：
--
作者：
G. Jones;M. G. Jones;M. Wilson;M. Brunori;A. Colosimo;P. Sarti
在CO存在下，用停流分光光度法和流动闪蒸技术研究了二亚硫酸盐还原细胞色素c氧化酶(EC 1.9.3.1)的反应。在酶中存在的两个血红素基团中，与细胞色素α相关的是第一个被还原的。本文报道了S2O4(2-)和SO2.-阴离子还原多种氧化还原蛋白(细胞色素c、星星花青素和天青素)的二级速率常数，并与细胞色素c氧化酶的测定结果进行了比较。这些结果从细胞色素C氧化酶电子进入位置的可及性和电荷分布的角度进行了讨论。
The reduction of cytochrome c oxidase (EC 1.9.3.1) by dithionite was investigated by stopped-flow spectrophotometry and flow-flash techniques in the presence of CO. Of the two haem groups present in the enzyme, that associated with cytochrome alpha is the first reduced. The second-order rate constants for reduction of a number of redox proteins (cytochrome c, stellacyanin and azurin) by the S2O4(2-) and SO2.- anions are reported, and the values are compared with those determined for cytochrome c oxidase. These results are discussed in terms of the accessibility and charge distribution of the electron-entry site of cytochrome c oxidase.