Overexpression of SGR Results in Oxidative Stress and Lesion-mimic Cell Death in Rice Seedlings
Overexpression of SGR Results in Oxidative Stress and Lesion-mimic Cell Death in Rice Seedlings
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DOI：
10.1111/j.1744-7909.2011.01037.x
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发表时间：
2011-05-01
影响因子：
11.4
通讯作者：
Wu, Guojiang
中科院分区：
文献类型：
--
作者：
Jiang, Huawu;Chen, Yaping;Wu, Guojiang
据认为，保持绿色水稻 (SGR) 基因参与光捕获复合物的解聚以及随后衰老过程中叶绿素和脱辅基蛋白的分解。在这项研究中，我们发现SGR（Ov-SGR）的过度表达导致单线态氧和其他活性氧的产生，并在水稻幼苗的叶片上产生叶绿素依赖性区域细胞死亡表型。使用 Affymetrix 水稻基因芯片进行的转录组分析表明，Ov-SGR 水稻幼苗表现出许多单线态氧反应的迹象。所确定的基因及其相关的生化途径提供了关于水稻植物如何在分子和生理水平上响应单线态氧的见解。
It is thought that the Stay Green Rice (SGR) gene is involved in the disaggregation of the light harvesting complex and in the subsequent breakdown of chlorophyll and apo-protein during senescence. In this study, we found that overexpression of SGR (Ov-SGR) resulted in the generation of singlet oxygen and other reactive oxygen species and produced a chlorophyll-dependent regional cell death phenotype on leaves of rice seedlings. Transcriptome analyses using Affymetrix Rice GeneChips revealed that Ov-SGR rice seedlings exhibited a number of signs of singlet oxygen response. The genes and their associated biochemical pathways identified provide an insight into how rice plants respond to singlet oxygen at the molecular and physiologic level.