Nanostructure analysis using spatially modulated illumination microscopy
Nanostructure analysis using spatially modulated illumination microscopy
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DOI：
10.1038/nprot.2007.399
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Birk, Udo J.
中科院分区：
文献类型：
--
作者：
Baddeley, David;Batram, Claudia;Birk, Udo J.
我们描述了使用空间调制照明（SMI）显微镜来估计荧光标记的细胞纳米结构的大小（和/或位置），包括对仪器及其处理的简要介绍。SMI显微镜的原理设置将被介绍，以解释成功的纳米结构分析所需的措施，然后给出样品制备，数据采集和评估的步骤。该方案从已经附着在盖玻片上的细胞开始。这里列出的方案和持续时间是固定标本的典型;然而，可以实现更快的数据采集和体内测量。
We describe the usage of the spatially modulated illumination (SMI) microscope to estimate the sizes (and/or positions) of fluorescently labeled cellular nanostructures, including a brief introduction to the instrument and its handling. The principle setup of the SMI microscope will be introduced to explain the measures necessary for a successful nanostructure analysis, before the steps for sample preparation, data acquisition and evaluation are given. The protocol starts with cells already attached to the cover glass. The protocol and duration outlined here are typical for fixed specimens; however, considerably faster data acquisition and in vivo measurements are possible.