Cytosolic distribution of villin in M cells from mouse Peyer's patches correlates with the absence of a brush border
Cytosolic distribution of villin in M cells from mouse Peyer's patches correlates with the absence of a brush border
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DOI：
10.1053/gast.1996.v110.pm8566599
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发表时间：
1996-02-01
期刊：
影响因子：
29.4
通讯作者：
Pringault, E
中科院分区：
文献类型：
--
作者：
Kerneis, S;Bogdanova, A;Pringault, E
背景与目的：Peyer's斑块的滤泡相关上皮（FAE）主要由两种细胞类型组成：具有刷状支架的吸收性肠细胞和没有这种根尖特化的M细胞。为了研究有争议的M细胞的个体发生（间充质细胞与上皮细胞），研究了组织特异性细胞骨架蛋白的表达模式，细胞骨架蛋白是细胞起源的标志物，在上皮细胞的特定形状和刷状边界组装中起着至关重要的作用。方法：采用免疫细胞化学和电镜法测定小鼠FAE细胞角蛋白、波形蛋白和绒毛蛋白的定位。结果：上皮特异性细胞角蛋白在吸收性肠细胞和M细胞中均有表达，而在小鼠FAE中未检测到波形蛋白。绒毛蛋白是一种组织特异性的肌动蛋白结合蛋白，在两种细胞类型中均有表达。该蛋白在缺乏刷状边界的FAE细胞和上皮内充满淋巴细胞的细胞中具有不寻常的细胞质分布。结论：M细胞中绒毛蛋白的存在和波形蛋白的缺失支持了M细胞的肠道起源。绒毛蛋白的细胞质分布为M细胞提供了新的鉴定标准，并反映了f -肌动蛋白网络的重组，这与M细胞无法组装刷状边界有关。
Background & Aims: The follicle-associated epithelium (FAE) of Peyer's patches mainly consists of two cell types: absorptive enterocytes with a brush bolder and M cells without this apical specialization. To study the controversial ontogeny of M cells (mesenchymal vs. epithelial origin), the expression pattern of tissue-specific cytoskeletal proteins, markers of cell origin that play a crucial role in the specific shape of epithelial cells and brush border assembly, was investigated. Methods: The localization of cytokeratins, vimentin, and villin was determined on mouse FAE using immunocytochemistry and electron microscopy. Results: Epithelial-specific cytokeratins were expressed in both absorptive enterocytes and M cells, whereas vimentin was not detected in mouse FAE. Villin, a tissue-specific, actin-binding protein of the brush border, was expressed in the two cell types. This protein had an unusual cytoplasmic distribution in FAE cells lacking a brush border and in cells having an intraepithelial pocket filled with lymphocytes. Conclusions: The presence of villin and the absence of vimentin in M cells support the intestinal origin of M cells. The cytoplasmic distribution of villin provides a new identification criteria for M cells and reflects the reorganization of the F-actin network, which correlates with the inability of M cells to assemble a brush border.