TISSUE DISTRIBUTION OF THE DEC-205 PROTEIN THAT IS DETECTED BY THE MONOCLONAL-ANTIBODY NLDC-145 .1. EXPRESSION ON DENDRITIC CELLS AND OTHER SUBSETS OF MOUSE LEUKOCYTES
TISSUE DISTRIBUTION OF THE DEC-205 PROTEIN THAT IS DETECTED BY THE MONOCLONAL-ANTIBODY NLDC-145 .1. EXPRESSION ON DENDRITIC CELLS AND OTHER SUBSETS OF MOUSE LEUKOCYTES
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DOI：
10.1006/cimm.1995.1109
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发表时间：
1995-06-01
影响因子：
4.3
通讯作者：
STEINMAN, RM
中科院分区：
文献类型：
--
作者：
INABA, K;SWIGGARD, WJ;STEINMAN, RM
多个研究小组先前的研究表明，mAb NLDC-145主要与树突状细胞（DC）和胸腺皮质的上皮细胞反应。我们最近报道，这种单克隆抗体识别DEC-205，一个205-kDa的完整的膜糖蛋白与一个独特的氨基末端序列，并提出了一个兔多克隆抗体纯化的DEC-205具有更高的亲和力，印迹抗原比原来的单克隆抗体。在这里，我们利用多克隆和NLDC-145来重新评估DEC-205在白细胞上的表达和功能。通过细胞荧光照相术，源自表皮（朗格汉斯细胞）和源自增殖的骨髓祖细胞（BMDC）的DC表达高水平（2- 3log）的DEC-205，而新鲜分离的脾DC包含两个亚群，大多数（80%）以低水平（小于或等于1 log）染色，其余为中等水平（1.5log）。DEC-205表位对胰蛋白酶敏感，但在培养中再生。居民和炎症性腹腔巨噬细胞不表达抗原，除了少量的巯基乙酸盐引起的细胞。来自脾、淋巴结、骨髓、血液和腹腔液的B细胞表达的DEC-205水平比BMDC上的DEC-205水平低10- 50倍。骨髓前B细胞和前B细胞不表达DEC-205。多克隆抗DEC-205未能抑制体外DC刺激初级混合白细胞反应，或体内局部移植物对宿主反应，其中将亲本T细胞注射到F1小鼠中。因此，DEC-205在白细胞上的表达比以前认识到的更广泛，但其功能仍不清楚。(C)出版社：Academic Press
Prior studies by a variety of groups demonstrated that the mAb NLDC-145 reacted primarily with dendritic cells (DCs) and the epithelial cells of the thymic cortex. We recently reported that this mAb recognizes DEC-205, a 205-kDa integral membrane glycoprotein with a unique amino-terminal sequence, and raised a rabbit polyclonal antibody to purified DEC-205 with higher affinity for the blotted antigen than the original mAb. Here we utilize both the polyclonal and NLDC-145 to reassess the expression and function of DEC-205 on leukocytes. By cytofluorography, DCs derived from the epidermis (Langerhans cells) and from proliferating bone marrow progenitors (BMDCs) expressed high levels (2-3 logs) of DEC-205, while freshly isolated spleen DCs comprised two subsets, most (80%) staining at low levels (less than or equal to 1 log), the remainder moderately (1.5 logs). DEC-205 epitopes were sensitive to trypsin, but were regenerated in culture. Resident and inflammatory peritoneal macrophages did not express the antigen, except for small amounts on thioglycollate-elicited cells. B cells from spleen, lymph node, bone marrow, blood, and peritoneal fluid expressed levels of DEC-205 that were 10- to 50-fold lower than those on BMDCs. Marrow pro- and pre-B cells did not express DEC-205. Polyclonal anti-DEC-205 failed to inhibit either stimulation of a primary mixed leukocyte reaction by DCs in vitro, or a local graft vs host response in vivo, where parental T cells were injected into F1 mice. DEC-205 is therefore more broadly expressed on leukocytes than previously appreciated, but its function remains unclear. (C) 1995 Academic Press, Inc.