GPR56 is highly expressed in neural stem cells but downregulated during differentiation
GPR56 is highly expressed in neural stem cells but downregulated during differentiation
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GPR56 在神经干细胞中高表达，但在分化过程中下调
DOI：
10.1097/wnr.0b013e32832c92d7
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发表时间：
2009-07-01
期刊：
影响因子：
1.7
通讯作者：
Zhang, Lijun
中科院分区：
文献类型：
--
作者：
Bai, Yun;Du, Liying;Zhang, Lijun
G蛋白偶联受体56（GPR 56）在脑发育和肿瘤发生中起重要作用。cDNA数据表明，GPR 56有潜力成为神经干细胞（NSC）或神经祖细胞（NPC）的标志物。然而，GPR 56蛋白在人NSC/NPCs中的表达未被探索。使用特异性抗体和免疫化学，我们发现，GPR 56是高度表达的nestin阳性的NSC/NPC在脑室/脑室下区的人类和小鼠胎脑，并在培养的神经球来自人类和小鼠胎脑。在分化的神经球培养物中，GPR 56蛋白的下调早于巢蛋白。GPR 56蛋白的丢失在分化良好的胶质细胞酸性蛋白阳性星形胶质细胞和III-微管蛋白阳性神经元中也很明显。我们的数据表明，GPR 56可用作神经细胞谱系内的NSC/NPC标记物，特别是与巢蛋白组合。
The G-protein-coupled receptor 56 (GPR56) plays important roles in brain development and tumorigenesis. cDNA data suggest that GPR56 has potential to become a neural stem cell (NSC) or neural progenitor cell (NPC) marker. However, expression of GPR56 protein in human NSC/NPCs was not explored. Using specific antibodies and immunochemistry, we showed that GPR56 was highly expressed in nestin-positive NSC/NPCs in the ventricular/subventricular zone of human and mouse fetal brains, and in cultured neurospheres derived from both human and mouse fetal brains. Downregulation of GPR56 protein occurred earlier than that of nestin in differentiating neurosphere cultures. Loss of GPR56 protein was also evident in well-differentiated glial fibrillary acidic protein-positive astrocytes and &bgr;III-tubulin-positive neurons. Our data suggest that GPR56 can be used as an NSC/NPC marker within the neural cell lineage, especially in combination with nestin.