Carbonylated proteins are eliminated during reproduction in C-elegans
Carbonylated proteins are eliminated during reproduction in C-elegans
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DOI：
10.1111/j.1474-9726.2010.00625.x
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发表时间：
2010-12-01
期刊：
影响因子：
7.8
通讯作者：
Aguilaniu, Hugo
中科院分区：
文献类型：
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作者：
Goudeau, Jerome;Aguilaniu, Hugo
在许多物种中，氧化损伤蛋白质随着年龄的增长而积累（Stadtman（1992）Science 257，1220-1224）。这意味着在繁殖时必须重置损伤。为了可视化这种重置在蛔虫秀丽隐杆线虫，一种新的免疫荧光技术，允许检测原位羰基化蛋白质的开发。该技术的应用表明，在C. elegans生殖这种净化在卵母细胞成熟时突然发生在生殖系内。令人惊讶的是，生殖细胞明显比周围的体细胞组织更氧化。因为已经提出了不同的机制来解释酵母和小鼠中的损伤消除（Aguilaniu等人（2003）Science 299，1751-1753; Hernebring等人（2006）Proc Natl Acad Sci USA 103，7700-7705），所以测试了蠕虫和这些系统之一之间可能的共同机制。结果表明，与酵母不同（Aguilaniu等人（2003）Science 299，1751-1753; Erjavec等人（2008）Proc Natl Acad Sci USA 105，18764-18769），蠕虫中羰基化蛋白的消除不需要存在长寿保证基因SIR-2.1。然而，与小鼠中的发现类似（Hernebring等（2006）Proc Natl Acad Sci USA 103，7700-7705），在C.优雅的因此，氧化损伤的蛋白质在蠕虫的繁殖过程中通过蛋白酶体被消除。这一发现表明，在生殖过程中受损蛋白质的重置是保守的，因此验证了C。elegans作为研究损伤消除的分子基础的模型。
P>Oxidatively damaged proteins accumulate with age in many species (Stadtman (1992) Science257, 1220-1224). This means that damage must be reset at the time of reproduction. To visualize this resetting in the roundworm Caenorhabditis elegans, a novel immunofluorescence technique that allows the detection of carbonylated proteins in situ was developed. The application of this technique revealed that carbonylated proteins are eliminated during C. elegans reproduction. This purging occurs abruptly within the germline at the time of oocyte maturation. Surprisingly, the germline was markedly more oxidized than the surrounding somatic tissues. Because distinct mechanisms have been proposed to explain damage elimination in yeast and mice (Aguilaniu et al. (2003) Science299, 1751-1753; Hernebring et al. (2006) Proc Natl Acad Sci USA103, 7700-7705), possible common mechanisms between worms and one of these systems were tested. The results show that, unlike in yeast (Aguilaniu et al. (2003) Science299, 1751-1753; Erjavec et al. (2008) Proc Natl Acad Sci USA105, 18764-18769), the elimination of carbonylated proteins in worms does not require the presence of the longevity-ensuring gene, SIR-2.1. However, similar to findings in mice (Hernebring et al. (2006) Proc Natl Acad Sci USA103, 7700-7705), proteasome activity in the germline is required for the resetting of carbonylated proteins during reproduction in C. elegans. Thus, oxidatively damaged proteins are eliminated during reproduction in worms through the proteasome. This finding suggests that the resetting of damaged proteins during reproduction is conserved, therefore validating the use of C. elegans as a model to study the molecular basis of damage elimination.