A novel 7-hypoxia-related long non-coding RNA signature associated with prognosis and proliferation in melanoma
A novel 7-hypoxia-related long non-coding RNA signature associated with prognosis and proliferation in melanoma
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DOI：
10.3892/mmr.2022.12771
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发表时间：
2022-08-01
影响因子：
3.4
通讯作者：
Chen, Aijun
中科院分区：
文献类型：
--
作者：
Luo, Yi;Li, Tinghao;Chen, Aijun
缺氧相关的长链非编码RNA（lncRNA）是肿瘤预后不良的重要指标。本研究旨在探讨黑色素瘤与缺氧相关lncRNAs的潜在关系。黑色素瘤患者的转录组和临床数据从癌症基因组图谱数据库下载。采用Pearson相关性检验和单因素考克斯分析筛选与预后相关的lncRNA。结果，构建了基于7个lncRNA表达的缺氧相关lncRNA标签，其中一个不利的[MIR 205宿主基因（MIR 205 HG）]和六个有利的（T细胞受体β可变区11-2，HLA-DQB 1反义RNA 1，AL365361.1，AC004847.1，泛素特异性肽酶30反义RNA 1和AC022706.1）lncRNA作为黑素瘤的预后因子。黑色素瘤患者根据获得的风险评分分为高风险组和低风险组。进行生存分析以评估本风险模型的预后价值。使用基因集富集分析探索了与本签名相关的潜在肿瘤相关生物学途径。CIBERSORT算法证明了缺氧相关lncRNA在调节肿瘤浸润免疫细胞中的重要作用。从我们中心收集的临床样本部分证实了我们的发现。细胞计数试剂盒-8和流式细胞术测定表明，抑制MIR 205 HG表达后，黑色素瘤细胞的增殖受到抑制。通过蛋白质印迹法检测经典Wnt/β-连环蛋白信号通路的指标。本研究表明MIR 205 HG可部分通过经典的Wnt/beta-catenin信号通路促进黑色素瘤细胞增殖。这些发现表明7-低氧相关的lncRNA特征可以作为黑色素瘤预后的新预测因子。
Hypoxia-related long non-coding RNAs (lncRNAs) are important indicators of the poor prognosis of cancers. The present study aimed to explore the potential relationship between melanoma and hypoxia-related lncRNAs. The transcriptome and clinical data of patients with melanoma were downloaded from The Cancer Genome Atlas database. The prognostic hypoxia-related lncRNAs were screened out using Pearson's correlation test and univariate Cox analysis. As a result, a hypoxia-related-lncRNA signature based on the expression of 7 lncRNAs was constructed, with one unfavourable [MIR205 host gene (MIR205HG)] and six favourable (T cell receptor beta variable 11-2, HLA-DQB1 antisense RNA 1, AL365361.1, AC004847.1, ubiquitin specific peptidase 30 antisense RNA 1 and AC022706.1) lncRNAs as prognostic factors for melanoma. Patients with melanoma were divided into high- and low-risk groups based on the risk score obtained. Survival analyses were performed to assess the prognostic value of the present risk model. Potential tumour-associated biological pathways associated with the present signature were explored using gene set enrichment analysis. The CIBERSORT algorithm demonstrated the important role of the hypoxia-related lncRNAs in regulating tumour-infiltrating immune cells. Clinical samples collected from our center partly confirmed our findings. Cell Counting Kit-8 and flow cytometry assays indicated the suppression of proliferation of melanoma cells following inhibition of MIR205HG expression. Indicators of the canonical Wnt/beta-catenin signalling pathway were detected by western blotting. The present study demonstrated that MIR205HG could promote melanoma cell proliferation partly via the canonical Wnt/beta-catenin signalling pathway. These findings indicated a 7-hypoxia-related-lncRNA signature that can serve as a novel predictor of melanoma prognosis.