Characterization of DNA Methylomic Signatures in Induced Pluripotent Stem Cells During Neuronal Differentiation.
Characterization of DNA Methylomic Signatures in Induced Pluripotent Stem Cells During Neuronal Differentiation.
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DOI：
10.3389/fcell.2021.647981
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发表时间：
2021
影响因子：
5.5
通讯作者：
Lunnon K
中科院分区：
文献类型：
--
作者：
Imm J;Pishva E;Ali M;Kerrigan TL;Jeffries A;Burrage J;Glaab E;Cope EL;Jones KM;Allen ND;Lunnon K
在发育过程中，从多能状态分化导致全局表观遗传变化，尽管在基于诱导多能干细胞的神经元模型中发生这种变化的程度尚未得到广泛表征。在本研究中，诱导的多能干细胞集落（33 Qn 1系）在四个时间点分化并收集，使用Illumina Infinium人类甲基化EPIC BeadChip阵列评估DNA甲基化。使用数据驱动的轨迹推断方法研究了分化过程中发生的DNA甲基化的动态变化。我们鉴定了大量的Bonferroni显著位点，这些位点在神经元分化过程中表现出DNA甲基化的进行性改变。基因-基因相互作用网络分析确定了60个密集连接的基因，这些基因对神经元的分化有影响，其中STAT 3是连接性最高的基因。
In development, differentiation from a pluripotent state results in global epigenetic changes, although the extent to which this occurs in induced pluripotent stem cell-based neuronal models has not been extensively characterized. In the present study, induced pluripotent stem cell colonies (33Qn1 line) were differentiated and collected at four time-points, with DNA methylation assessed using the Illumina Infinium Human Methylation EPIC BeadChip array. Dynamic changes in DNA methylation occurring during differentiation were investigated using a data-driven trajectory inference method. We identified a large number of Bonferroni-significant loci that showed progressive alterations in DNA methylation during neuronal differentiation. A gene–gene interaction network analysis identified 60 densely connected genes that were influential in the differentiation of neurons, with STAT3 being the gene with the highest connectivity.
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影响因子：
7.7
作者：
Gutierrez-Arcelus M;Lappalainen T;Montgomery SB;Buil A;Ongen H;Yurovsky A;Bryois J;Giger T;Romano L;Planchon A;Falconnet E;Bielser D;Gagnebin M;Padioleau I;Borel C;Letourneau A;Makrythanasis P;Guipponi M;Gehrig C;Antonarakis SE;Dermitzakis ET
通讯作者：
Dermitzakis ET
影响因子：
7
作者：
Spiers H;Hannon E;Schalkwyk LC;Smith R;Wong CC;O'Donovan MC;Bray NJ;Mill J
通讯作者：
Mill J
DOI：
10.1073/pnas.1820843116
发表时间：
2020-09-22
影响因子：
11.1
作者：
McEwen, Lisa M.;O'Donnell, Kieran J.;Kobor, Michael S.
通讯作者：
Kobor, Michael S.
影响因子：
23.9
作者：
通讯作者：
--
儿童早期发育的表观遗传学。
影响因子：
4.7
作者：
Murgatroyd C;Spengler D
通讯作者：
Spengler D