Nano-sized collagen I molecules enhanced the differentiation of rat mesenchymal stem cells into cardiomyocytes
Nano-sized collagen I molecules enhanced the differentiation of rat mesenchymal stem cells into cardiomyocytes
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DOI：
10.1002/jbm.a.34589
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发表时间：
2013-10-01
影响因子：
4.9
通讯作者：
Kuo, Shyh Ming
中科院分区：
文献类型：
--
作者：
Kang, Pei-Leun;Chen, Chih-Hao;Kuo, Shyh Ming
本研究的目的是研究纳米尺寸的胶原蛋白I分子（纳米颗粒或纳米纤维）和5-氮杂胞苷（5-aza）处理，以提高大鼠间充质干细胞（MSC）向心肌表型在体外分化的能力。将第二代MSC与纳米尺寸的胶原蛋白I分子共培养24小时，然后用10 M 5-aza处理24小时。结果表明，细胞的大小显着增加，并获得一个扁平的，三角形的形态后，与纳米尺寸的胶原蛋白I分子和5-氮杂处理。细胞通过形成肌管样结构与相邻细胞连接。与仅用5-aza处理的MSC组相比，用纳米尺寸的胶原蛋白I原纤维额外处理MSC在3天培养时显著增加了两种转录因子加塔-4（增加12.6倍）和Nkx 2.5（增加4.8倍）的表达。此外，与仅用5-aza处理的MSC组相比，用纳米尺寸的胶原纤维预处理的MSC显著增加了肌钙蛋白I、β-肌球蛋白重链和心脏肌动蛋白的心脏基因的表达（全部，p < 0.01或更好）。这些结果表明，培养MSC与纳米尺寸的胶原蛋白I分子，这可能是作为支架或可溶性蛋白质成分，导致基因表达的改变，并影响分化的命运与5-氮诱导。(c)2013 Wiley Periodicals，Inc. J Biomed Mater Res Part A：101A：2808-2816，2013.
The aim of this study was to investigate the ability of nano-sized collagen I molecules (nanoparticles or nanofibrils) and a 5-azacytidine (5-aza) treatment to enhance the differentiation of rat mesenchymal stem cells (MSCs) toward a cardiomyogenic phenotype in vitro. Second passaged MSCs were cocultured with nano-sized collagen I molecules for 24 h and then treated with 10 M 5-aza for 24 h. The results demonstrated that the size of the cells increased significantly and acquired a flattened, triangular-shaped morphology after treatment with nano-sized collagen I molecules and 5-aza. The cells are connecting with adjoining cells by forming myotube-like structures. Additional treatment of the MSCs with nano-sized collagen I fibrils significantly increased two transcription factors GATA-4 (12.6-fold increase) and Nkx2.5 (4.8-fold increase) expressions compared with MSC groups treated only with 5-aza at 3-day culturing. Furthermore, MSCs pretreated with nano-sized collagen fibrils significantly increased the expressions of cardiac genes of troponin I, -myosin heavy chain, and cardiac -actin compared with MSC groups treated only with 5-aza (all, p < 0.01 or better). These results indicate that culturing MSCs with nano-sized collagen I molecules, which may act as scaffolds or soluble protein ingredients, leads to alterations in gene expression and affects the differentiation fate induced with 5-aza. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 2808-2816, 2013.