Plasmon-induced photothermal cell-killing effect of gold colloidal nanoparticles on epithelial carcinoma cells
Plasmon-induced photothermal cell-killing effect of gold colloidal nanoparticles on epithelial carcinoma cells
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DOI：
10.1039/b907524k
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发表时间：
2009-01-01
影响因子：
3.1
通讯作者：
Ahmmad, Bashir
中科院分区：
文献类型：
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作者：
Abdulla-Al-Mamun, Md;Kusumoto, Yoshihumi;Ahmmad, Bashir
金胶体纳米粒子是通过在水中对金金属板进行液体激光烧蚀以及通过柠檬酸还原 HAuCl4 中心点 4H(2)O 来制备的。具有等离子体带的金胶体纳米粒子强烈吸收光，并将其转化为光热能。这种光热能对上皮癌细胞产生细胞毒性作用。有趣的是，我们发现纳米粒子的尺寸和形状在体外光热过程中被光改变。将宫颈癌细胞系（HeLa 细胞）与胶体金纳米粒子一起孵育，然后暴露在带有紫外线和热截止滤光片的 400-600 nm 连续可见光下。通过该程序观察到明显的细胞杀伤作用。在没有金胶体纳米颗粒的情况下，只有少量细胞被光热破坏。
Gold colloidal nanoparticles were prepared by the liquid laser ablation of a gold metal plate in water and also by the citrate reduction of HAuCl4 center dot 4H(2)O. The gold colloidal nanoparticles with the plasmonic band strongly absorb light, which is converted to the photothermal energy. This photothermal energy gives a cytotoxic effect on epithelial carcinoma cells. Interestingly, we found that the size and shape of the nanoparticles are changed by light during the photothermal process in vitro. The cervical carcinoma cell line (HeLa cell) was incubated with the colloidal gold nanoparticles and then exposed to continuous visible light at 400-600 nm with UV- and heat-cutoff filters. The distinct cell-killing effect was observed by this procedure. In the absence of the gold colloidal nanoparticles, only a small amount of cells were photothermally destroyed.