Decrease in ovarian platelet-activating factor during ovulation in the gonadotropin-primed immature rat.
Decrease in ovarian platelet-activating factor during ovulation in the gonadotropin-primed immature rat.
复制标题
促性腺激素引发的未成熟大鼠排卵期间卵巢血小板活化因子减少。
DOI：
10.1095/biolreprod41.1.104
复制
发表时间：
1989
影响因子：
3.6
通讯作者：
Okamura,H
中科院分区：
文献类型：
--
作者：
Espey,LL;Tanaka,N;Woodard,DS;Harper,MJ;Okamura,H
血小板活化因子（PAF）是一种具有生物活性的磷脂，在急性炎症反应和组织损伤时局部释放。由于有证据表明哺乳动物排卵的生化事件类似于炎症反应，本研究的目的是确定卵巢PAF水平是否在排卵期间发生变化。在促性腺激素引发的25日龄Wistar大鼠排卵过程中，在2小时的时间间隔，卵巢摘除，匀浆，并提取脂质。将提取物进行薄层色谱（TLC），并将与PAF共迁移的硅胶部分重新提取并测定PAF活性。通过生物测定法测量PAF（合成PAF的fmole当量），该生物测定法基于浸提液等分试样从家兔全血中分离并用[3 H]-5-羟色胺预标记的血小板中释放[3 H]-5-羟色胺的能力。用人绒毛膜促性腺激素（hCG）处理后2小时，卵巢PAF水平从6.67 ± 0.77下降到4.27 ± 0.45 fmoles/mg卵巢，下降了36%（p <0.01），hCG处理后4小时，卵巢PAF水平又下降了14%。卵巢PAF在hCG后24小时内保持在这一降低的水平。在hCG后1 h给予吲哚美辛（5 mg/rat，sc）或依泊司坦（5 mg/rat，sc）可阻止排卵，但两种药物均不影响卵巢PAF的下降。初步试验表明，卵巢的脂质提取物也含有PAF抑制剂，其在TLC板上与PAF共迁移。与PAF相似，hCG处理后4 h内卵巢中脂溶性抑制剂减少（p <0.05）。（250字处删节）
Platelet-activating factor (PAF) is a biologically active phospholipid that is released locally during acute inflammatory reactions and tissue injury. Since there is evidence that the biochemical events of mammalian ovulation resemble an inflammatory reaction, the objective of this study was to determine whether ovarian levels of PAF change during ovulation. At 2-h intervals during the ovulatory process in gonadotropin-primed 25-day-old Wistar rats, the ovaries were extirpated, homogenized, and extracted for lipids. The extracts were subjected to thin-layer chromatography (TLC), and the portion of the silica gel that comigrated with PAF was re-extracted and assayed for PAF activity. The PAF was measured (in fmole equivalents of synthetic PAF) by a bioassay based on the capacity of aliquots of the extracts to release [3H]-serotonin from platelets isolated from whole blood of rabbits and prelabeled with [3H]-serotonin. The ovarian level of PAF decreased (p less than 0.01) by 36% from 6.67+/-0.77 to 4.27+/-0.45 fmoles/mg ovary by 2 h after treatment with human chorionic gonadotropin (hCG), and it declined another 14% by 4 h after hCG. The ovarian PAF remained at this reduced level for up to 24 h after hCG. The administration of indomethacin (5 mg/rat, sc) or epostane (5 mg/rat, sc) at 1 h after hCG prevented ovulation, but neither drug affected the decline in ovarian PAF. Preliminary tests showed that the lipid extracts from the ovaries also contained PAF inhibitor (s) that comigrated with PAF on the TLC plates. Similar to PAF, the lipid-soluble inhibitor (s) decreased (p less than 0.05) in the ovaries within 4 h after hCG treatment.(ABSTRACT TRUNCATED AT 250 WORDS)