Genotypic evolution and antigenic drift of H9N2 influenza viruses in China from 1994 to 2008
Genotypic evolution and antigenic drift of H9N2 influenza viruses in China from 1994 to 2008
复制标题
1994-2008年中国H9N2流感病毒基因型进化和抗原漂移
DOI：
10.1016/j.vetmic.2010.05.010
复制
发表时间：
2010-12-15
影响因子：
3.3
通讯作者：
Liu, Jinhua
中科院分区：
文献类型：
--
作者：
Sun, Yipeng;Pu, Juan;Liu, Jinhua
H9N2流感病毒自1994年以来就在中国流行，但自2004年以来一直没有对中国北方地区的H9N2流感病毒进行系统调查。本文利用我们2003-2008年从中国北方地区家禽和猪中分离到的22种病毒的序列，结合公共数据库中的序列，分析了1994年至2008年中国H9N2流感病毒的演变。 研究结果表明，中国的H9N2病毒经历了广泛的重组，新的基因型不断出现。在330种病毒中，观察到54种基因型，其中包括19种以前未被识别的新基因型，主要基因型进一步分为5个系列（BJ/94系列、G1 BG系列、F/98系列和Aq系列）。人们认识到这些系列中不同的流行病学和生物学特征。 BJ/94和F/98系列病毒在中国南方和北方都有传播，而其他三个系列病毒主要在中国南方发现。 BJ/94系列流感病毒在2000年之前在中国占主导地位，并逐渐被F/98系列病毒取代，自2004年起成为主要病毒。在研究期间至少可以鉴定出5个抗原组，其中2002年至2003年间可能发生显着的抗原漂移。动物实验表明F/98系列病毒能够在鸡中更有效地复制和传播 比 BJ/94 系列病毒。 H9N2 流感病毒在中国的持续进化凸显了整个地区 H9N2 流感病毒监测对于帮助大流行预测和预防的重要性。 (C) 2010 Elsevier B.V. 保留所有权利。
H9N2 influenza viruses have been circulating in China since 1994, but a systematic investigation of H9N2 in northern China has not been undertaken since 2004. Here, using the sequences of 22 viruses we isolated from poultry and pigs in northern China during 2003-2008, in combination with sequences available in a public database, we analyzed the evolution of H9N2 influenza viruses in China from 1994 to 2008. Our findings demonstrated that the H9N2 viruses in China underwent extensive reassortment, and novel genotypes continued to emerge. Among 330 viruses, 54 genotypes were observed including 19 novel genotypes that have not been recognized before, and major genotypes were further divided into five series (BJ/94-, G1 BG-, F/98- and Aq-series). Different epidemiological and biological features among these series were recognized. The BJ/94- and F/98-series viruses were circulating in both southern and northern China, while the other three series viruses were mainly detected in southern China. BJ/94-series influenza viruses predominated in China before 2000 and were gradually replaced by F/98-series viruses that became the predominant viruses since 2004. At least five antigenic groups could be identified over the study period, during which a significant antigenic drift likely occurred between 2002 and 2003. Animal experiments demonstrated that F/98-series viruses were able to replicate and transmit more effectively in chickens than BJ/94-series viruses. The continuing evolution of H9N2 influenza viruses in China emphasizes the importance of H9N2 influenza virus surveillance throughout this region to aid pandemic prediction and prevention. (C) 2010 Elsevier B.V. All rights reserved.