Reductive assays for S-nitrosothiols:: Implications for measurements in biological systems
Reductive assays for S-nitrosothiols:: Implications for measurements in biological systems
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DOI：
10.1006/bbrc.1998.9688
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发表时间：
1998-11-27
影响因子：
3.1
通讯作者：
Gaston, B
中科院分区：
文献类型：
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作者：
Fang, KZ;Ragsdale, NV;Gaston, B
在许多组织中都发现了具有生物活性的 SNO。我们推测 SNO 在将 NO3- 还原为 NO 的传统测定中可能会被错误识别。 S-亚硝基硫醇暴露于 HCl 中的饱和 VCl3、乙酸中的 1% KI、光解或 He 中的 CuCl 和 CSH； NO 通过化学发光法测量，S-亚硝基硫醇在 VCl3 中很容易检测到，但在 KI 中则不然。 CuCl/半胱氨酸的还原呈线性（r(2) = 1.0，n = 6），对 10 pmol 敏感，并被 HgCl2 消除；它没有检测到 NO2-NO3- 或 3-硝基酪氨酸。 S-亚硝基硫醇相当于人血清中 VCl3 测定的 2.9% 的 NOx，其中
Bioactive SNOs are found in many tissues. We speculated SNOs might be misidentified in conventional assays which reduce NO3- to NO. S-Nitrosothiols were exposed to saturated VCl3 in HCl, 1% KI in acetic acid, photolysis, or CuCl and CSH in He; NO was measured by chemiluminescence, S-Nitrosothiols were readily detected in VCl3 but not in KI. Reduction in CuCl/cysteine was linear (r(2) = 1.0, n = 6), sensitive to 10 pmol, and eliminated by HgCl2; it did not detect NO2- NO3- or 3-nitrotyrosine. S-Nitrosothiols represented similar to 2.9% of NOx assayed by VCl3 in human serum, of which