Osteoblastic lysosome plays a central role in mineralization
Osteoblastic lysosome plays a central role in mineralization
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DOI：
10.1126/sciadv.aax0672
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发表时间：
2019-07-01
期刊：
影响因子：
13.6
通讯作者：
Murakami, Shinya
中科院分区：
文献类型：
--
作者：
Iwayama, Tomoaki;Okada, Tomoko;Murakami, Shinya
Mineralization is the most fundamental process in vertebrates. It is predominantly mediated by osteoblasts, which secrete mineral precursors, most likely through matrix vesicles (MVs). These vesicular structures are calcium and phosphate rich and contain organic material such as acidic proteins. However, it remains largely unknown how intracellular MVs are transported and secreted. Here, we use scanning electron-assisted dielectric microscopy and super-resolution microscopy for assessing live osteoblasts in mineralizing conditions at a nanolevel resolution. We found that the calcium-containing vesicles were multivesicular bodies containing MVs.They were transported via lysosome and secreted by exocytosis. Thus, we present proof that the lysosome transports amorphous calcium phosphate within mineralizing osteoblasts.
