Aberrant ascus genotypes from crosses involving mutants at the g locus in Sordaria fimicola

Aberrant ascus genotypes from crosses involving mutants at the g locus in Sordaria fimicola
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涉及 Sordaria fimicola g 位点突变体杂交的异常子囊基因型

DOI:
10.1017/s0016672300015263
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发表时间:
1974
期刊:
影响因子:
1.5
通讯作者:
H. L. K. Whitehouse
H. L. K. Whitehouse
中科院分区:
生物学4区
文献类型:
--
作者:
Y. Kitani;H. L. K. Whitehouse

文献摘要

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本文给出了4500多个ASCI基因座的遗传型别,这些基因座在灰(G)孢子色基因座上表现出异常分离,并存在外部标记,存在于g基因座突变体和野生型之间,或涉及孢子颜色不同的一对g基因座突变体(g基因座突变体的范围从灰色到透明)。这种颜色差异使ASCI在两点杂交和野生型(黑色)孢子中检测到突变孢子颜色的异常分离。在总共涉及7个初级和2个次级g基因座突变体的21个一点杂交中,2700个异常ASCI被分成14个可识别的ASCU基因型,而在两点杂交中,来自10个两两组合的1830个ASCI(满分1044分)被归类为150个可区分的ASCUS基因型。
The genotypes are given of over 4500 asci showing aberrant segregation at the grey ( g ) spore colour locus in Sordaria fimicola from crosses, with outside markers present, between g locus mutants and wild type, or involving pairs of g locus mutants that differed in spore colour ( g locus mutants range from grey to hyaline). This colour difference enabled asci with aberrant segregation of the mutant spore colours to be detected in the two-point crosses, as well as those with wild-type (black) spores. From a total of 21 one-point crosses involving altogether seven primary and two secondary g locus mutants, 2700 aberrant asci have been grouped into the 14 ascus genotypes that can be recognized, and in the two-point crosses 1830 asci (1044 fully scored) from 10 pairwise combinations of the mutants have been classified into the 150 ascus genotypes that can be distinguished.