Wasp Venom Possesses Potential Therapeutic Effect in Experimental Models of Rheumatoid Arthritis
Wasp Venom Possesses Potential Therapeutic Effect in Experimental Models of Rheumatoid Arthritis
复制标题
黄蜂毒液对类风湿关节炎实验模型具有潜在的治疗作用
DOI：
10.1155/2020/6394625
复制
发表时间：
2020-04-06
影响因子：
--
通讯作者：
Yang, Zhi-Bin
中科院分区：
文献类型：
--
作者：
Gao, Yuan;Yu, Wan-Xin;Yang, Zhi-Bin
风湿性关节炎（RA）是一种自身免疫性疾病。胡蜂毒是我国云南景颇族民间传统药物，治疗类风湿性关节炎。本研究旨在探讨黄蜂毒对实验性大鼠类风湿关节炎症状的改善作用。我们建立了SD大鼠II型胶原诱导的关节炎（CIA）模型，并检查了炎症和自身免疫反应的抑制作用。以大鼠足跖肿胀度、组织病理学评分和组织病理学改变评价WV的抗炎作用。以外周血中IL-6、TNF-α、IL-1 β水平及炎性细胞数为指标，观察其抗炎作用。采用流式细胞仪检测大鼠脾脏T细胞亚群比例的变化，同时测定脏器指数和血清免疫球蛋白水平。结果表明，WV（0.125、0.25、0.5mg/kg）能显著减轻CIA大鼠足肿胀和关节炎评分（P
Rheumatoid arthritis (RA) is an autoimmune disease. Wasp venom (WV), which is considered as a traditional folk medicine in Jingpo nationality in Yunnan, China, relieves rheumatoid arthritis. The current study aimed to investigate the effect of wasp venom ameliorating rheumatoid arthritis symptoms in experimental rats. We established a model of type II collagen- (CII-) induced arthritis (CIA) in SD rats and examined the inhibition of inflammation and autoimmune response. The antiarthritic effects of WV were evaluated through the paw swelling, and histopathological score and histopathology changes of the affected paw were assessed. The anti-inflammation effects were assayed by the level of IL-6, TNF-alpha, IL-1 beta, and the number of inflammatory cells in peripheral blood. The alteration of the T cell subset ratio in the spleen of rats was detected by flow cytometry, and at the same time, the viscera index and immune serum globulin levels were evaluated. The results suggested that various doses of WV (0.125, 0.25, and 0.5 mg/kg) significantly alleviated paw swelling and arthritis score in CIA rats with the untreated control (P