A CULTURE‐MEDIUM AIDING THE IDENTIFICATION OF VERTICILLIUM ALBO‐ATRUM AND V. DAHLIAE
A CULTURE‐MEDIUM AIDING THE IDENTIFICATION OF VERTICILLIUM ALBO‐ATRUM AND V. DAHLIAE
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一种有助于鉴定黄萎病和大丽花的培养基
DOI：
10.1111/j.1365-3059.1960.tb01147.x
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发表时间：
1960
期刊：
影响因子：
2.7
通讯作者：
P. Talboys
中科院分区：
文献类型：
--
作者：
P. Talboys
本文介绍了用培养基鉴定黄萎病菌和大丽花黄萎病菌的方法。培养基中加入浓缩西梅提取物100毫升，乳糖5克，Difco酵母提取物1克，琼脂30克，蒸馏水共1000毫升。介质的氢电位调整为5.8-6.0。两种植物的轮状分生孢子通常在三到四天内出现。大丽花黄萎病菌的微菌核在接种平板后约5天开始发育，虽然在这个阶段通常肉眼不可见，但在显微镜下可以很容易地看到菌丝的侧芽。白斑黄萎病菌落下侧的宏观外观具有纤维状特征，与大丽花黄萎病的砂砾状外观截然不同，甚至肉眼可见。
This article describes the use of a medium in identifying Verticillium albo-atrum and Verticillium dahliae. The medium contains concentrated prune extract 100 milliliter, lactose five grams, Difco yeast extract one gram, agar 30 grams, and distilled water to make up 1,000 milliliter. The potential of hydrogen of the medium is adjusted to 5.8-6.0. Verticillate conidiophores of both species are usually apparent within three to four days. Microsclerotia of Verticillium dahliae begin to develop about five days after inoculation of the plate, and though they are not usually visible to the naked eye at this stage, the lateral budding of the hyphae can easily be seen under the microscope. The macroscopic appearance of the underside of the Verticillium albo-atrum colony is characteristically fibrous and quite distinct from the gritty appearance of Verticillium dahliae, even to the naked eye.