Nuclei purified from cauliflower mosaic virus-infected turnip leaves contain subgenomic, covalently closed circular cauliflower mosaic virus DNAs.
Nuclei purified from cauliflower mosaic virus-infected turnip leaves contain subgenomic, covalently closed circular cauliflower mosaic virus DNAs.
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从感染花椰菜花叶病毒的萝卜叶中纯化的细胞核含有亚基因组、共价闭合的环状花椰菜花叶病毒 DNA。
DOI：
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发表时间：
1983
影响因子：
14.9
通讯作者：
T. Guilfoyle
中科院分区：
文献类型：
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作者：
N. Olszewski;T. Guilfoyle
从花椰菜花叶病毒（CaMV）感染的芜菁叶片中分离的细胞核除了含有基因组长度的CaMV DNA外，还含有亚基因组CaMV DNA种类。这些亚基因组CaMV DNA种类以共价闭合环（形式I）、松弛环（形式II）和线性（形式III）分子存在。亚基因组I型DNA种类的大小范围从基因组长度的约10%到接近基因组长度。这些亚基因组DNA物种出现在感染克隆CaMV DNA的组织中，表明它们迅速出现，并且没有在CaMV连续繁殖的病毒群体中积累。CaMV基因组的特定区域似乎没有被优先删除以形成亚基因组CaMV DNA种类。至少有三种不同的亚基因组物种似乎优先积累在从感染组织分离的细胞核中。这些丰富的亚基因组CaMV DNA种类中的两种是I型，另一种是III型。一些亚基因组CaMV DNA种类似乎是微型染色体。
Nuclei isolated from cauliflower mosaic virus (CaMV) infected turnip leaves contain subgenomic CaMV DNA species in addition to the genome length CaMV DNA. These subgenomic CaMV DNA species are present as covalently closed circles (form I), relaxed circles (form II) and linear (form III) molecules. The subgenomic form I DNA species range in size from about 10% of genome length to nearly genome length. These subgenomic DNA species appear in tissue infected with cloned CaMV DNA, indicating that they arise rapidly and have not accumulated in the virus population from serial propagation of CaMV. No specific region of the CaMV genome appears to be preferentially deleted to form the subgenomic CaMV DNA species. At least three distinct subgenomic species appear to accumulate preferentially in nuclei isolated from infected tissue. Two of these abundant subgenomic CaMV DNA species are form I and the other one is form III. Some of the subgenomic CaMV DNA species appear to be minichromosomes.