Nuclear import time and transport efficiency depend on importin beta concentration.
Nuclear import time and transport efficiency depend on importin beta concentration.
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DOI：
10.1083/jcb.200605053
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发表时间：
2006-09-25
期刊：
影响因子：
--
通讯作者：
Musser SM
中科院分区：
文献类型：
--
作者：
Yang W;Musser SM
虽然许多组件和双向运输所需的反应步骤，通过核膜（NE）的特点，通过核孔复合物（NPC）的货物迁移的机制和控制仍然知之甚少。单分子荧光显微镜被用来跟踪货物的运动之前，期间和之后，他们与NPC的相互作用。在低的输入素β浓度下，与NPC相互作用的信号依赖性货物中约有一半穿过NE移位，表明核输入效率为50%。在高β输入浓度下，输入效率增加到约80%，转运速度增加约7倍。高浓度的β-输入也提高了非信号依赖性货物的转运速度和输入效率。这些结果表明，最大核质转运速度可以通过importin β浓度调节至少10倍，因此提示了调节穿过NE的货物运输速度的潜在机制。
Although many components and reaction steps necessary for bidirectional transport across the nuclear envelope (NE) have been characterized, the mechanism and control of cargo migration through nuclear pore complexes (NPCs) remain poorly understood. Single-molecule fluorescence microscopy was used to track the movement of cargos before, during, and after their interactions with NPCs. At low importin β concentrations, about half of the signal-dependent cargos that interacted with an NPC were translocated across the NE, indicating a nuclear import efficiency of ∼50%. At high importin β concentrations, the import efficiency increased to ∼80% and the transit speed increased approximately sevenfold. The transit speed and import efficiency of a signal-independent cargo was also increased by high importin β concentrations. These results demonstrate that maximum nucleocytoplasmic transport velocities can be modulated by at least ∼10-fold by the importin β concentration and therefore suggest a potential mechanism for regulating the speed of cargo traffic across the NE.
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影响因子：
7.8
作者：
DWORETZKY, SI;FELDHERR, CM
通讯作者：
FELDHERR, CM
影响因子：
7.8
作者：
Lyman, SK;Guan, TL;Gerace, L
通讯作者：
Gerace, L
DOI：
10.1073/pnas.92.14.6532
发表时间：
1995-07-03
影响因子：
11.1
作者：
MOROIANU, J;HIJIKATA, M;RADU, A
通讯作者：
RADU, A
DOI：
10.1073/pnas.96.17.9622
发表时间：
1999-08-17
影响因子：
11.1
作者：
Nachury, MV;Weis, K
通讯作者：
Weis, K
影响因子：
11.4
作者：
BISCHOFF, FR;KREBBER, H;PONSTINGL, H
通讯作者：
PONSTINGL, H