Analysis of combinatorial loss-of-function mutants in the Arabidopsis ethylene receptors reveals that the ers1 etr1 double mutant has severe developmental defects that are EIN2 dependent
Analysis of combinatorial loss-of-function mutants in the Arabidopsis ethylene receptors reveals that the ers1 etr1 double mutant has severe developmental defects that are EIN2 dependent
复制标题
DOI：
10.1105/tpc.013060
复制
发表时间：
2003-09-01
期刊：
影响因子：
11.6
通讯作者：
Bleecker, AB
中科院分区：
文献类型：
--
作者：
Hall, AE;Bleecker, AB
拟南芥中的乙烯反应受ETR受体家族控制。受体作为下游信号转导组分的负调节剂起作用，并且基于序列相似性而分为两个不同的亚家族。为了阐明受体亚型之间的功能冗余水平，产生了包括新分离的ers 1 -2等位基因的组合突变株系。基于黄化幼苗生长响应，测试的所有突变体组合表现出一些组成型乙烯响应性，但也保持对外源乙烯的响应性，表明所有五种受体亚型都可以有助于信号传导，并且没有一种受体亚型是必需的。另一方面，光生长的幼苗和成人ERS 1 ETR 1双突变体表现出严重的表型，如微型莲座大小，延迟开花，不育，揭示了一个独特的作用，在光生长的植物亚科I受体。将ein 2功能丧失突变引入ers 1 etr 1双突变株系中导致植物表型复制ein 2单突变体，表明在ers 1 etr 1双突变体中观察到的所有表型都依赖于EIN 2。
Ethylene responses in Arabidopsis are controlled by the ETR receptor family. The receptors function as negative regulators of downstream signal transduction components and fall into two distinct subfamilies based on sequence similarity. To clarify the levels of functional redundancy between receptor isoforms, combinatorial mutant lines were generated that included the newly isolated ers1-2 allele. Based on the etiolated seedling growth response, all mutant combinations tested exhibited some constitutive ethylene responsiveness but also remained responsive to exogenous ethylene, indicating that all five receptor isoforms can contribute to signaling and no one receptor subtype is essential. On the other hand, light-grown seedlings and adult ers1 etr1 double mutants exhibited severe phenotypes such as miniature rosette size, delayed flowering, and sterility, revealing a distinct role for subfamily I receptors in light-grown plants. Introduction of an ein2 loss-of-function mutation into the ers1 etr1 double mutant line resulted in plants that phenocopy ein2 single mutants, indicating that all phenotypes observed in the ers1 etr1 double mutant are EIN2 dependent.