Geographically specific heteroplasmy of mitochondrial DNA in the seaweed, Fucus serratus (Heterokontophyta: Phaeophyceae, Fucales)
Geographically specific heteroplasmy of mitochondrial DNA in the seaweed, Fucus serratus (Heterokontophyta: Phaeophyceae, Fucales)
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DOI：
10.1111/j.1365-294x.2004.02128.x
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发表时间：
2004-05-01
影响因子：
4.9
通讯作者：
Olsen, JL
中科院分区：
文献类型：
--
作者：
Coyer, JA;Hoarau, G;Olsen, JL
据报道，在脊椎动物、无脊椎动物、担子菌和一些被子植物中，同一生物体内存在不止一种类型的线粒体DNA（线粒体DNA异质性），但从未在海洋（大型）藻类中存在过。我们利用单链构象多态性 (SSCP) 检测了潮间岩藻（Fucus serratus）线粒体中 nad11 基因 135 个碱基对 (bp) 区域的序列差异。来自 Blushoj（丹麦）和 Oskarshamn（瑞典）的 70 名和 22 名个体分别显示单倍型 2、3 和 4（= mtDNA 异质性），而来自西班牙、法国、爱尔兰、冰岛和挪威的 24 名个体中仅发现单倍型 2。由于布鲁绍伊和奥斯卡港是末次盛冰期（距今 18 000-20 000 年）期间最后从冰盖中露出来的地区之一，地理上特定的异质性可能代表了奠基者效应，因此是了解冰河时代后重新殖民作用的一个有价值的标志。地理特异性异质性在基于 mtDNA 序列的系统发育地理学研究中也具有重要意义。
The presence of more than one type of mitochondrial DNA within the same organism (mtDNA heteroplasmy) has been reported in vertebrates, invertebrates, basidiomycetes and some angiosperms, but never in marine (macro)algae. We examined sequence differences in a 135-base pair (bp) region of the nad11 gene in mitochondria of the intertidal rockweed, Fucus serratus, using single-strand conformation polymorphism (SSCP). Each of 70 and 22 individuals from Blushoj (Denmark) and Oskarshamn (Sweden), respectively, displayed haplotypes 2, 3, and 4 (= mtDNA heteroplasmy), whereas only haplotype 2 was found in each of 24 individuals from locations in Spain, France, Ireland, Iceland and Norway. As Blushoj and Oskarshamn were among the last areas to emerge from ice cover during the Last Glacial Maximum (18 000-20 000 years BP), the geographically specific heteroplasmy may represent a founder effect and therefore, a valuable marker for understanding the role of post-Ice Age recolonization. Geographically specific heteroplasmy also has important implications in phylogeographical studies based on mtDNA sequences.