Comparison of two cytochromes P-450 from Candida maltosa: primary structures, substrate specificities and effects of their expression in Saccharomyces cerevisiae on the proliferation of the endoplasmic reticulum.
Comparison of two cytochromes P-450 from Candida maltosa: primary structures, substrate specificities and effects of their expression in Saccharomyces cerevisiae on the proliferation of the endoplasmic reticulum.
复制标题
麦芽糖念珠菌两种细胞色素 P-450 的比较：初级结构、底物特异性及其在酿酒酵母中表达对内质网增殖的影响。
DOI：
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发表时间：
1991
影响因子：
6.6
通讯作者：
H. Müller
中科院分区：
文献类型：
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作者：
W. Schunck;F. Vogel;B. Gross;E. Kärgel;S. Mauersberger;K. Köpke;C. Gengnagel;H. Müller
克隆、测序并表达了麦芽假丝酵母两种不同形式的细胞色素P-450，分别命名为P-450 Cm1和P-450 Cm2。推导的氨基酸序列同源性约为55%。在酿酒酵母中的表达导致形成完整的微粒体P-450系统，催化正十六烷和月桂酸的羟化反应，具有明显不同的底物偏好。在产生P-450的酿酒酵母细胞中观察到内质网的大量增殖。根据P-450的表达形式，出现了明显排列的成对膜，并占据了相当大的细胞质区域。P-450Cm1的免疫电子显微镜显示，表达的蛋白高度集中在这些新形成的膜结构中。
cDNAs were cloned, sequenced and expressed which encode two different cytochrome P-450 forms of the alkane-assimilating yeast Candida maltosa, designated as P-450Cm1 and P-450Cm2. The amino acid sequences deduced were about 55% identical. Expression in Saccharomyces cerevisiae resulted in the formation of intact microsomal P-450 systems catalyzing the hydroxylation of n-hexadecane and lauric acid with significantly different substrate preferences. A massive proliferation of the endoplasmic reticulum was observed in the S. cerevisiae cells which produced P-450. Depending on the P-450 form expressed, distinctly organized stacks of paired membranes appeared and occupied considerable areas of the cytoplasm. As shown by immunoelectron microscopy for P-450Cm1, the protein expressed was highly concentrated within these newly formed membrane structures.