Cell-type-specific expression of transforming growth factor-alpha in the mouse uterus during the peri-implantation period.
Cell-type-specific expression of transforming growth factor-alpha in the mouse uterus during the peri-implantation period.
复制标题
围着床期间小鼠子宫中转化生长因子-α的细胞类型特异性表达。
DOI：
10.1095/biolreprod45.2.365
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发表时间：
1991
影响因子：
3.6
通讯作者：
Dey,SK
中科院分区：
文献类型：
--
作者：
Tamada,H;Das,SK;Andrews,GK;Dey,SK
采用免疫组织化学、原位杂交和Northern blot杂交技术检测小鼠子宫中转化生长因子-α (TGF-α)在着床期的时间和细胞类型特异性表达。TGF-α（免疫组化）及其mRNA（原位杂交）在妊娠第1 - 4天（第1天=阴道塞）和许多基质细胞（第3天和第4天）中共定位，表明这些细胞是着床前TGF-α合成的主要部位。Northern blotting显示，第4天子宫总RNA中TGF-α mRNA水平升高，与表达该基因的基质细胞募集一致。在着床后（5-8天），着床部位蜕膜中mRNA和蛋白的共定位表明蜕膜间质细胞合成TGF-α。虽然原位杂交显示胚胎在第5 - 8天存在mRNA，但免疫染色仅在第5和6天在胚胎中发现。上述结果提示，着床期子宫和胚胎中TGF-α的表达可能参与了胚胎发育、子宫着床准备和脱卵过程。
Immunohistochemistry as well as in situ and Northern blot hybridization were employed to determine temporal and cell-type-specific expression of transforming growth factor-α (TGF-α) in the mouse uterus during the peri-implantation period. The co-localization of TGF-α (by immunohistochemistry) with its mRNA (by in situ hybridization) in the luminal and glandular epithelia on Days 1–4 of pregnancy (Day 1 = vaginal plug) and also in many stromal cells on Days 3 and 4 indicates that these cells are the primary sites of TGF-α synthesis during the preimplantation period. The higher levels of TGF-α mRNA in total uterine RNA on Day 4, as shown by Northern blotting, is consistent with the recruitment of stromal cells expressing this gene. During the post-implantation period (Days 5–8), the co-localization of the mRNA and protein in the decidua at the implantation sites suggests that the decidualizing stromal cells synthesize TGF-α. Although in situ hybridization showed the presence of mRNA in embryos on Days 5–8, immunostaining was noted in the embryo only on Days 5 and 6. These results suggest that uterine and embryonic expression of TGF-α during the peri-implantation period could be involved in embryonic development, preparation of the uterus for implantation, and decidualization.