CD8+ thymic lymphocytes express reduced levels of CD8beta and increased interferon gamma in cats perinatally infected with the JSY3 molecular clone of feline immunodeficiency virus.
CD8+ thymic lymphocytes express reduced levels of CD8beta and increased interferon gamma in cats perinatally infected with the JSY3 molecular clone of feline immunodeficiency virus.
复制标题
在围产期感染猫免疫缺陷病毒 JSY3 分子克隆的猫中，CD8 胸腺淋巴细胞表达的 CD8β 水平降低，而干扰素 γ 水平增加。
DOI：
10.1089/088922200750006083
复制
发表时间：
2000
影响因子：
1.5
通讯作者：
Johnson,CM
中科院分区：
文献类型：
--
作者：
Orandle,MS;Crawford,PC;Levy,JK;Udoji,R;Papadi,GP;Ciccarone,T;Mergia,A;Johnson,CM
猫免疫缺陷病毒 (FIV) 的生物分离株会导致受感染猫胸腺内激活的单阳性 CD8+ (SP CD8+) 淋巴细胞相对扩增。在本研究中，对新生儿时接种 FIV 致病性分子克隆 JSY3 的猫的胸腺 SP CD8+ 淋巴细胞进行了分析，该克隆先前源自野生型生物分离株 FIVNCSU-1 (NCSU-1)。四只猫腹腔内接种 NCSU-1，并与 11 只猫接种 JSY3 进行比较。对五只同窝和年龄相匹配的对照猫进行腹膜内假接种。接种后 12 至 16 周，通过 RT-PCR 对新鲜分离的胸腺细胞和外周血单核细胞 (PBMC) 中的干扰素-γ (IFN-γ) mRNA 进行定量。与假接种对照相比，13 只接种 FIV 的猫中有 13 只的胸腺细胞和 PBMC 中 IFN-γ mRNA 的数量增加了 10 倍以上。 IFN-γ mRNA 与磁性分选的 CD8+PBMC 和单阳性 (SP) CD8+ 胸腺细胞共富集。表达 IFN-γ mRNA 的细胞位于胸腺血管周围区，沿着皮质髓质连接处，并邻近淋巴滤泡。胸腺 SP CD8+ 细胞的扩增与 CD8α+/β 阴性和 CD8α+/β 表型的增加相关，后者群体类似于先前报道的具有 FIV 抑制活性的记忆/效应外周血细胞。从这些数据我们得出结论，JSY3 和 NCSU-1 在胸腺和外周血 CD8+ 细胞中诱导相似的表型变化。因此，JSY3 对体内胸腺具有致病性，并且可用于确定该儿科 AIDS 模型中 CD8+ 细胞反应的决定因素。
Biological isolates of feline immunodeficiency virus (FIV) cause a relative expansion of activated single-positive CD8+(SP CD8+) lymphocytes within the thymus of infected cats. In this study, thymic SP CD8+lymphocytes were analyzed from cats inoculated as neonates with a pathogenic molecular clone of FIV, JSY3, which was previously derived from the wild-type biological isolate FIVNCSU-1(NCSU-1). Four cats were inoculated intraperitoneally with NCSU-1 and compared with 11 cats inoculated with JSY3. Five control cats matched in litter and age were administered an intraperitoneal sham inoculum. Between 12 and 16 weeks postinoculation, interferon-γ (IFN-γ) mRNA was quantified by RT-PCR in freshly isolated thymocytes and peripheral blood mononuclear cells (PBMCs). The quantity of IFN-γ mRNA was increased more than 10-fold in thymocytes and PBMCs of 13 of 13 FIV-inoculated cats as compared with the sham-inoculated controls. IFN-γ mRNA coenriched with magnetically sorted CD8+PBMCs and single-positive (SP) CD8+thymocytes. Cells expressing IFN-γ mRNA were located within the thymic perivascular zone, along the corticomedullary junction, and adjacent to lymphoid follicles. The expansion of thymic SP CD8+cells was associated with an increase in CD8α+/βnegand CD8α+/βlophenotypes, the latter population resembling a previously reported memory/effector peripheral blood cell with FIV suppressor activity. From these data we conclude that JSY3 and NCSU-1 induce similar phenotypic changes in thymic and peripheral blood CD8+cells. Thus, JSY3 is pathogenic for the thymusin vivoand will be useful for defining determinants of the CD8+cell response in this pediatric AIDS model.