Existence of Novel Phylotypes of Nitrite-Dependent Anaerobic Methane-Oxidizing Bacteria in Surface and Subsurface Sediments of the South China Sea
Existence of Novel Phylotypes of Nitrite-Dependent Anaerobic Methane-Oxidizing Bacteria in Surface and Subsurface Sediments of the South China Sea
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DOI：
10.1080/01490451.2014.917742
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发表时间：
2015-01-02
影响因子：
2.3
通讯作者：
Gu, Ji-Dong
中科院分区：
文献类型：
--
作者：
Chen, Jing;Jiang, Xi-Wen;Gu, Ji-Dong
亚硝酸盐依赖型厌氧甲烷氧化（n-damo）是近年来发现的一种新的微生物过程，由Methylomirabilisoxyfera产生，具有独特的好氧内氧化途径，但海洋环境中n-damo细菌的研究尚未见报道。M.以16 SrRNA和pmoA基因为生物标志物，采用PCR扩增技术，首次从南海表层和次表层沉积物中成功提取到了类尖孢菌（oxyfera-like）基因序列。大多数M。从南海沉积物中扩增的16 S rRNA基因组成了一个新的类群，与淡水环境中的16 S rRNA基因完全不同，与pmoA基因的信息在遗传学上是一致的。研究结果表明，在海洋沉积物中存在n-damo，并表明海洋沉积物中含有不同于淡水的n-damo类型。这一发现扩展了我们对n-damo细菌在海洋生态系统中分布的认识，并暗示了它们对海洋C和N循环的潜在贡献。
Nitrite-dependent anaerobic methane oxidation (n-damo) is a recently discovered new microbial process performed by the Candidatus Methylomirabilis oxyfera with an unusual intra-aerobic pathway, but there is no report about n-damo bacteria in marine environments. M. oxyfera-like sequences were successfully retrieved for the first time from both surface and subsurface ocean sediments of the South China Sea (SCS) using both 16S rRNA and pmoA genes as biomarkers and PCR amplification in this study. The majority of M. oxyfera-like 16S rRNA gene-based PCR amplified sequences from the SCS sediments formed a new group distinctively different from those detected in freshwater habitats and the information is consistent phylogenetically with those obtained from the pmoA gene. This study showed the existence of n-damo in ocean sediments and suggests that marine sediments harbor n-damo phylotypes different from those in the freshwater. This finding here expands our understanding on the distribution of n-damo bacteria to marine ecosystem and implies their potential contribution to the marine C and N cycling.