ISOLATION OF THE TRANSPOSABLE MAIZE CONTROLLING ELEMENTS AC AND DS
ISOLATION OF THE TRANSPOSABLE MAIZE CONTROLLING ELEMENTS AC AND DS
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DOI：
10.1016/0092-8674(83)90226-x
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
SHURE, M
中科院分区：
文献类型：
--
作者：
FEDOROFF, N;WESSLER, S;SHURE, M
含有Wx基因座部分的限制性内切酶片段是从在该基因座处具有由控制元件激活子（Ac）和解离（Ds）引起的插入突变的菌株中克隆的。有证据表明，遗传上定义的Ac元件对应于4.3 kb的插入，而2 Ds元件对应于4.1 kb和2.0 kb的插入，都在Wx转录单位的3 "末端附近。4.1 kb的Ds与Ac元件几乎完全同源，不同之处在于小于0.2 kb的中心缺失。2.0 kb Ds元件与Ac元件的末端同源。与Ac元件的末端同源的序列以许多拷贝存在于所检查的基因组中，而与克隆的Ac元件的中心同源的序列有10个或更少的拷贝。在Wx基因座的Ac元件可以从结构上区别于基因组中的其他Ac样序列。
Restriction endonuclease fragments containing part of the Waxy (Wx) locus were cloned from strains with insertion mutations at the locus caused by the controlling elements Activator (Ac) and Dissociation (Ds). Evidence is presented that the genetically defined Ac element corresponds to a 4.3 kb [kilobase] insertion, while the 2 Ds elements correspond to 4.1 kb and 2.0 kb insertions, all near the 3'' end of the Wx transcription unit. The 4.1 kb Ds is almost completely homologous to the Ac element, differing by a central deletion of less than 0.2 kb. The 2.0 kb Ds element is homologous to the ends of the Ac element. Sequences homologous to the ends of the Ac element are present in many copies in the genomes examined, while there are 10 or fewer copies of a sequence with homology to the center of the cloned Ac element. The Ac element at the Wx locus can be distinguished structurally from the other Ac-like sequences in the genome.