New members of the Drosophila Myc transcription factor subfamily revealed by a genome-wide examination for basic helix-loop-helix genes
New members of the Drosophila Myc transcription factor subfamily revealed by a genome-wide examination for basic helix-loop-helix genes
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DOI：
10.1016/s0925-4773(01)00360-4
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发表时间：
2001-06-01
影响因子：
2.6
通讯作者：
Haenlin, M
中科院分区：
文献类型：
--
作者：
Peyrefitte, S;Kahn, D;Haenlin, M
碱性螺旋-环-螺旋（bHLH）蛋白代表一类进化上保守的转录因子，已知其在动物胚胎发育期间的细胞决定和分化中发挥重要作用。使用PSI-BLAST策略对果蝇完整基因组序列进行详尽搜索后，我们发现了19个新基因，使果蝇基因组中编码bHLH的基因总数达到56个。这些新基因属于bHLH转录因子的不同亚家族，如Daughterless、Hairy-Enhancer of split、bHLH-PAS或bHLHZip亚家族。这些新基因的胚胎表达模式进行了原位杂交分析。通过寻找结构上密切相关的基序，我们发现了两个可能代表脊椎动物Mnt和Mix直向同源物的基因。与以前的报告一起，我们的数据表明，与神经发生和肌肉发生中涉及的网络类似，Myc相关基因的网络在整个进化过程中一直是全球保守的。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
The basic helix-loop-helix (bHLH) proteins represent an evolutionary conserved class of transcription factors that are known to play important roles in cell determination and differentiation during animal embryonic development. Following an exhaustive search of the complete Drosophila genome sequence using a PSI-BLAST strategy, we identified 19 new genes, bringing the total number of bHLH-encoding genes in the Drosophila genome to 56. These new genes belong to various subfamilies of bHLH transcription factors, such as the Daughterless, Hairy-Enhancer of split, bHLH-PAS or bHLHZip subfamilies. The embryonic expression pattern of each of these new genes has been analyzed by in situ hybridization. By looking for closely structurally related motifs, we found two genes that represent likely orthologues of vertebrate Mnt and Mix. Together with previous reports, our data suggest that, similar to networks involved in neurogenesis and myogenesis, the network of Myc-related genes has been globally conserved throughout evolution. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.