Effect of the proportion of organic material in bone on thermal decomposition of bone mineral: An investigation of a variety of bones from different species using thermogravimetric analysis coupled to mass spectrometry, high-temperature X-ray diffraction, and Fourier transform infrared spectroscopy
Effect of the proportion of organic material in bone on thermal decomposition of bone mineral: An investigation of a variety of bones from different species using thermogravimetric analysis coupled to mass spectrometry, high-temperature X-ray diffraction, and Fourier transform infrared spectroscopy
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DOI：
10.1007/s00223-004-0199-5
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发表时间：
2004-10-01
影响因子：
4.2
通讯作者：
Hukins, DWL
中科院分区：
文献类型：
--
作者：
Mkukuma, LD;Skakle, JMS;Hukins, DWL
与质谱（TGA-MS）相关的热重分析显示了质量的变化，并识别了材料加热时产生的气体。加热到600度，可以将骨骼样本分为有机物质比例高（鳕鱼胸骨、鹿角骨和鲸鱼的骨膜鳍骨）和低（海豚耳骨、鲸鱼鼓膜和鲸鱼耳骨）两类。在较高的温度下，骨头的矿物阶段分解。高温x射线衍射（HTXRD）结果表明，矿物（在800℃~ 1000℃的空气或氩气中）分解产生的主要固体是鹿角中的β -磷酸三钙（TCP）和羟基磷灰石（HAP），鲸鱼鼓膜中的CaO和HAP。在二氧化碳中，分解被延缓了，这表明在空气和氩气中观察到的变化是矿物中碳酸盐离子损失的结果。加热到不同温度的骨头的傅里叶变换红外光谱（FTIR）表明，二氧化碳的损失（由于碳酸盐离子的分解）伴随着氢氧化物离子的出现。这些结果可以用ca10 -x V-x((Ca))[(PO4)(6-x-y)(HPO4)(x)(CO3)(y)][(OH)(2-x-y)(CO3)(y)V-x((OH))]表示来解释，其中V-(Ca)和V-(OH)分别对应于钙和氢氧化物位点上的空位，2-x-y = 0.4。这个通式既适用于描述Ca/P摩尔比高、HPO42-含量低、CO32-含量高的成熟骨矿物（如鲸骨），也适用于描述未成熟骨矿物（如鹿角）。Ca/P较低，HPO42-较高，CO32-含量较低。
Thermogravimetric analysis linked to mass spectrometry (TGA-MS) shows changes in mass and identifies gases evolved when a material is heated. Heating to 600degreesC enabled samples of bone to be classified as having a high (cod clythrum, deer antler, and whale periotic fin bone) or a low (porpoise ear bone, whale tympanic bulla, and whale ear bone) proportion of organic material. At higher temperatures, the mineral phase of the bone decomposed. High temperature X-ray diffraction (HTXRD) showed that the main solids produced by decomposition of mineral (in air or argon at 800degreesC to 1000degreesC) were beta-tricalcium phosphate (TCP) and hydroxyapatite (HAP), in deer antler, and CaO and HAP, in whale tympanic bulla. In carbon dioxide, the decomposition was retarded, indicating that the changes observed in air and argon were a result of the loss of carbonate ions from the mineral. Fourier transform infrared (FTIR) spectroscopy of bones heated to different temperatures, showed that loss of carbon dioxide (as a result of decomposition of carbonate ions) was accompanied by the appearance of hydroxide ions. These results can be explained if the structure of bone mineral is represented byCa10-x V-x((Ca))[(PO4)(6-x-y)(HPO4)(x)(CO3)(y)][(OH)(2-x-y)(CO3)(y)V-x((OH))]where V-(Ca) and V-(OH) correspond to vacancies on the calcium and hydroxide sites, respectively, and 2-x-y = 0.4. This general formula is consistent in describing both mature bone mineral (i.e., whale bone), with a high Ca/P molar ratio, lower HPO42- content, and higher CO32- content, and immature bone mineral (i.e., deer antler)., with a low Ca/P ratio, higher HPO42- and lower CO32- content.