Phenotypic analysis and effects of sequential administration of activated canine lymphocytes on healthy beagles
Phenotypic analysis and effects of sequential administration of activated canine lymphocytes on healthy beagles
复制标题
DOI：
10.1292/jvms.70.581
复制
发表时间：
2008-06-01
影响因子：
1.2
通讯作者：
Fujinaga, Toru
中科院分区：
文献类型：
--
作者：
Hoshino, Yuki;Takagi, Satoshi;Fujinaga, Toru
我们试图为小动物医学中活化淋巴细胞疗法的评估积累基础数据。使用抗CD3抗体和人重组(hr)白细胞介素(IL)-2激活从健康狗收集的外周血单核细胞(PBMC)，并使用hr干扰素(IFN)-α和hr IL-2重新激活。将获得的细胞的特性与PBMC进行比较。通过培养，细胞数量增加了约 50 倍以上。 CD8(+)细胞比例显着增加，培养细胞的细胞毒性得到增强。此外，CD56 mRNA 水平趋于增加。通过该方法获得的细胞被证实为活化的淋巴细胞。此外，我们研究了将获得的细胞连续施用给健康狗的效果。通过连续给予活化的淋巴细胞，细胞增殖活性、CD4(+)细胞和CD8(+)细胞的比例以及血清IFN-γ浓度均有所增加，并且没有观察到严重的不良反应。因此，在健康狗中使用抗CD3抗体和IL2可以诱导活化的淋巴细胞，并且连续施用活化的淋巴细胞增强了受体的免疫力。
We attempted to accumulate the basic data for evaluation of activated lymphocyte therapy for small animal medicine. The peripheral blood mononuclear cells (PBMCs) collected from healthy dogs were activated using anti-CD3 antibody and human recombinant (hr) interleukin (lL)-2 and reactivated using hr interferon (IFN)-alpha and hr IL-2. The property of obtained cells was compared with PBMCs. The number of cells was shown to have increased approximately > 50-fold by cultivation. The proportion of CD8(+) cells was significantly increased, the cytotoxicity of the cultured cells was revealed to have been reinforced. Additionally, CD56 mRNA levels tended to have increased. The cells obtained by this method were confirmed to be activated lymphocytes. Furthermore, we investigated the effects of sequential administration of the obtained cells to healthy dogs. By sequential administration of the activated lymphocytes, the cell proliferative activity, proportion of CD4(+) cells and CD8(+) cells, and serum IFN-gamma concentration were shown to have increased, and no severe adverse effects were observed. Consequently, activated lymphocytes could be induced using anti-CD3 antibody and IL2 in healthy dogs, and sequential administration of activated lymphocytes reinforced the recipient's immunity.