Discs lost, a novel multi-PDZ domain protein, establishes and maintains epithelial polarity
Discs lost, a novel multi-PDZ domain protein, establishes and maintains epithelial polarity
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DOI：
10.1016/s0092-8674(00)80593-0
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发表时间：
1999-03-19
期刊：
影响因子：
64.5
通讯作者：
Bellen, HJ
中科院分区：
文献类型：
--
作者：
Bhat, MA;Izaddoost, S;Bellen, HJ
上皮细胞的极化依赖于一个分级过程，从而特定的膜相关蛋白成为专门的膜结构域的目标。在这里，我们描述了一种新的果蝇蛋白，光盘丢失（DLT），在细胞胚盘形成过程中胚胎上皮细胞的极化中起着至关重要的作用。在随后的发育阶段，DLT与顶端决定簇Crumbs（CRB）和侧向定位蛋白Neurexin IV（NRX IV）相互作用。dlt突变或双链RNA干扰导致CRB和NRX IV的异常定位，并导致上皮细胞极性的伴随损失。因此，DLT是建立和维持细胞极性所必需的，并参与定义顶膜和侧膜结构域的不同分子复合物。
Polarization of epithelial cells depends on a hierarchical process whereby specific membrane-associated proteins become targeted to specialized membrane domains. Here, we describe a novel Drosophila protein, Discs Lost (DLT), that plays a crucial role in the polarization of embryonic epithelia during cellular blastoderm formation. At subsequent stages of development, DLT interacts with the apical determinant Crumbs (CRB) and the laterally localized protein Neurexin IV (NRX IV). Mutations in dlt or double-stranded RNA interference lead to aberrant localization of CRB and NRX IV and cause a concomitant loss of epithelial cell polarity. Hence, DLT is required to establish and maintain cell polarity and participates in different molecular complexes that define apical and lateral membrane domains.