Isolation and characterization of a putative keratin-associated protein gene expressed in embryonic skin of mice.
Isolation and characterization of a putative keratin-associated protein gene expressed in embryonic skin of mice.
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在小鼠胚胎皮肤中表达的假定角蛋白相关蛋白基因的分离和表征。
DOI：
10.1046/j.1523-1747.1998.00241.x
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
N. Huh
中科院分区：
文献类型：
--
作者：
M. Takaishi;Y. Takata;T. Kuroki;N. Huh
胚胎小鼠皮肤从性交后 13 天 (dpc) 到 16 dpc 经历了显着的形态变化，即从 13.5 dpc 时的单层上皮细胞和周皮到 16.5 dpc 时几乎完全分化的具有毛囊雏形的分层上皮。利用RNA差异显示，我们分离出了一个参与小鼠表皮发育的基因。该基因暂时命名为4C32，编码197个氨基酸，其中包含6个带有CQ基序的10个氨基酸的同向重复序列。在大多数角蛋白相关蛋白家族中都可以看到带有 CQ 基序的重复结构，这些蛋白家族已知在毛囊中特异性表达。 4C32在15.5和16.5 dpc时在胚胎表皮的最外层表达，并在17.5 dpc时突然消失，表明4C32在周皮中表达。周皮是胚胎表皮的表层，已知在小鼠胚胎中 17 dpc 时会消失。在发育和成熟的舌头组织以及尾鳞中也检测到了 4C32 转录本，但在毛囊的任何阶段都没有检测到。
Embryonic mouse skin undergoes substantial morphologic changes from 13 days post-coitus (dpc) to 16 dpc, i.e., from simple layers of epithelial cells and periderm at 13.5 dpc to almost fully differentiated stratified epithelium with the rudiments of hair follicles at 16.5 dpc. Using RNA differential display, we isolated a gene involved in the development of mouse epidermis. This gene, tentatively designated as 4C32, encodes 197 amino acids containing six direct repeats of 10 amino acids with the CQ motif. The repetitive structure with the CQ motif is seen in most keratin-associated protein families, which are known to be specifically expressed in hair follicles. 4C32 is expressed in the outermost layer of the embryonic epidermis at 15.5 and 16.5 dpc, and abruptly disappears at 17.5 dpc, suggesting that 4C32 is expressed in the periderm. The periderm is a superficial layer of embryonic epidermis, and is known to disappear at 17 dpc in mouse embryos. The 4C32 transcripts were also detected in the developing and matured tongue tissues and in the tail scale, but not at any stage in hair follicles.
影响因子：
56.9
作者：
BONIFAS, JM;ROTHMAN, AL;EPSTEIN, EH
通讯作者：
EPSTEIN, EH