Effects of UV on the migration and function of epidermal antigen presenting cells
Effects of UV on the migration and function of epidermal antigen presenting cells
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DOI：
10.1016/s0027-5107(98)00186-9
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发表时间：
1998-11-09
影响因子：
2.3
通讯作者：
Muller, HK
中科院分区：
文献类型：
--
作者：
Dandie, GW;Clydesdale, GJ;Muller, HK
一段时间以来，在国际文献中已经确立了过度暴露于紫外线B光（UVB）后表皮朗格汉斯细胞（LC）的耗竭和伴随的皮肤免疫系统的抑制。我们的研究旨在确定这些现象是否是由于UVB暴露引发的LC迁移增加的直接结果。为了检验这一假设，建立了绵羊模型，其中将引流皮肤的限定区域的淋巴管插管，并连续收集向区域淋巴结迁移的细胞。通过间接免疫荧光和流式细胞术鉴定和计数这些收集物中的细胞群。这些实验表明，有一个显着的，剂量依赖性增加LC迁移率从绵羊皮肤暴露后，剂量的UVB光超过1个最小平均剂量（MED）。通过一系列平行实验，研究了正常绵羊和UVB照射绵羊的树突状细胞（DC）迁移的功能特性。为了测定它们，通过插管传入淋巴管收集富集的DC制剂，并在与自体外周血淋巴细胞孵育之前用抗原脉冲。抗原呈递的相对效率由DC诱导T细胞增殖的能力决定。我们的数据清楚地表明，有一个深刻的损失后，暴露于UVB光正常抗原呈递细胞功能。进行了各种实验以确定与迁移动力学和细胞功能的这些变化相关的机制。LC迁移从正常或UVB照射皮肤的电子显微镜检查已证明UVB暴露后的树突状过程的深刻损失。这为UVB暴露后皮肤免疫力的变化提供了可能的解释。(C)1998 Elsevier Science B.V.保留所有权利。
Depletion of epidermal Langerhans cells (LC) and the concomitant depression of the skin immune system after excessive exposure to ultraviolet B light (UVB) has been established in the international literature for some time. Our investigations were intended to determine whether or not these phenomena occurred as a direct result of increased LC migration being triggered by the UVB exposure. To test this hypothesis, a sheep model was established in which the lymphatic vessels draining a defined region of skin were cannulated and the cells migrating towards the regional lymph node continuously collected. Cell populations in these collections were identified and enumerated by indirect immunofluorescence and flow cytometry. These experiments showed there was a significant, dose-dependent increase in the rate of LC migration from sheep skin after exposure to doses of UVB light exceeding 1 minimal erythemal dose (MED). In a series of parallel experiments, the functional characteristics of dendritic cells (DC) migrating from normal or UVB irradiated sheep were studied. To assay them, enriched preparations of DC were collected via cannulated afferent lymphatic vessels and pulsed with antigen prior to incubation with autologous peripheral blood lymphocytes. The relative efficiency of antigen presentation was determined by the ability of DC to induce T cell proliferation. Our data clearly demonstrate that there is a profound loss of normal antigen-presenting cell function after exposure to UVB light. Various experiments were undertaken to determine the mechanism(s) associated with these changes in migration kinetics and cellular function. Electron microscopic examinations of LC migrating from normal or UVB irradiated skin have demonstrated a profound loss of dendritic processes after UVB exposure. This provides a possible explanation for the changes in skin immunity after UVB exposure. (C) 1998 Elsevier Science B.V. All rights reserved.