Effects of repeated applications of fungicide carbendazim on its persistence and microbial community in soil.
Effects of repeated applications of fungicide carbendazim on its persistence and microbial community in soil.
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DOI：
10.1016/s1001-0742(08)62248-2
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发表时间：
2009
影响因子：
6.9
通讯作者：
Y. Yu;X. Chu;Guohui Pang;Y. Xiang;H. Fang
中科院分区：
文献类型：
--
作者：
Y. Yu;X. Chu;Guohui Pang;Y. Xiang;H. Fang
多菌灵是一种系统性苯并咪唑杀菌剂，在一个生长季节内反复应用于防治包括土传疾病在内的植物病害。在实验室条件下进行了研究，以评估重复使用多菌灵对其持久性和土壤中微生物群落的影响。结果表明，多菌灵在土壤中的消解随使用频率的增加而加快。多菌灵的降解速率常数从0.074 d−显著增加到0.79 d−。重复给药4次，半衰期由9.3d缩短至0.9d。在第一次处理后，多菌灵对土壤微生物对碳源的利用没有明显的抑制作用，但在第二次、第三次和第四次处理后，平均井色发展(AWCD)略有增加。这表明土壤微生物在反复使用多菌灵后，开始适应多菌灵。多菌灵处理土壤微生物群落的Simpson指数和Shannon指数与对照土壤微生物群落的Simpson指数和Shannon指数基本一致，表明土壤微生物的丰富度和优势特征在重复施药后保持不变。然而，第1、2、3次添加多菌灵后，第21天的McIntosh指数显著高于对照，这表明土壤微生物的平衡被改变了，这是由于土壤中特定的多菌灵适应菌株的丰富。
Carbendazim, a systemic benzimidazole fungicide, is applied repeatedly to control plant diseases including soilborne diseases, over a growing season. Studies were carried out under laboratory conditions to assess the effects of repeated carbendazim applications on its persistence and microbial community in soil. The results indicate that dissipation of carbendazim in soil was accelerated with its application frequency. The degradation rate constant of carbendazim was increased significantly from 0.074 d−to 0.79 d−. The corresponding half-life was shorten markedly from 9.3 d to 0.9 d after four repeated applications. No significant inhibitory effect of carbendazim on soil microbial utilization of the carbon sources was observed after first treatment, but a slight increase in average well color development (AWCD) was shown after second, third, and fourth applications. It suggested that soil microorganisms become adapted to carbendazim after repeated application. Simpson and Shannon indexes of soil microbial community from carbendazim treated soil were also similar to those from the control soil, indicating that the richness and dominant character of soil microorganisms remain unchangeable after repeated application. However, after first, second, and third addition of carbendazim, McIntosh indexes on day 21 were significantly higher compared with the control, suggesting that balance of soil microorganisms was altered due to the enrichment of the specific carbendazim-adapting strains in soil.