Transcriptional activity of Pax3 is co-activated by TAZ
Transcriptional activity of Pax3 is co-activated by TAZ
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DOI：
10.1016/j.bbrc.2005.10.214
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发表时间：
2006-01-13
影响因子：
3.1
通讯作者：
Kurihara, H
中科院分区：
文献类型：
--
作者：
Murakami, M;Tominaga, J;Kurihara, H
Pax 3是一种在胚胎发育和人类疾病中发挥作用的转录因子。在酵母双杂交筛选中，我们以全长Pax 3为诱饵，从E10.5小鼠胚胎cDNA文库中分离到一个编码转录辅激活因子的克隆，该转录辅激活因子具有PDZ结合基序（TAZ）。TAZ与Pax 3的免疫共沉淀和核共定位表明它们的关联是功能相关的。原位杂交显示TAZ和Pax 3的表达在近轴中胚层、肢芽和神经管中部分重叠。在C2 C12成肌细胞和NIH 3 T3细胞中，TAZ增强了Pax 3在人工和小眼症相关转录因子启动子-荧光素酶构建体上的转录活性，表明TAZ可以作为Pax 3的共激活因子发挥作用。Pax 3和TAZ之间的功能相互作用可能为阐明Pax 3在胚胎发生过程中作为转录激活因子的机制提供线索。(c)2005年爱思唯尔公司All rights reserved.
Pax3 is a transcription factor which functions in embryonic development and human diseases. In a yeast two-hybrid screen with full-length Pax3 as bait, we isolated a clone encoding transcriptional co-activator with PDZ-binding motif (TAZ) from an E10.5 mouse embryo cDNA library. Co-immunoprecipitation and nuclear co-localization of TAZ with Pax3 suggest that their association is functionally relevant. In situ hybridization revealed TAZ and Pax3 expression to partially overlap in the paraxial mesoderm, limb buds, and the neural tube. In C2C12 myoblast cells and NIH3T3 cells, TAZ enhanced the transcriptional activity of Pax3 on artificial and microphthalmia-associated transcription factor promoter-luciferase constructs, suggesting that TAZ can function as a co-activator of Pax3. Functional interaction between Pax3 and TAZ may provide a clue to clarifying the mechanism by which Pax3 serves as a transcriptional activator during embryogenesis. (c) 2005 Elsevier Inc. All rights reserved.