Regrowth of zebrafish caudal fin regeneration is determined by the amputated length
Regrowth of zebrafish caudal fin regeneration is determined by the amputated length
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DOI：
10.1038/s41598-020-57533-6
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发表时间：
2020-01
影响因子：
4.6
通讯作者：
Toshiaki Uemoto;Gembu Abe;K. Tamura
中科院分区：
文献类型：
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作者：
Toshiaki Uemoto;Gembu Abe;K. Tamura
鱼有很强的再生鳍的能力，包括尾鳍。尾鳍截肢后，在其快速再生的过程中重建了原有的双叶形态。胚泡细胞的位置记忆是否像两栖动物肢体再生一样调控鳍形态的重建仍存在争议，在两栖动物肢体再生中，位于同一近端和远端水平的肢体胚芽细胞具有相同的位置一致性。对斑马鱼尾鳍再生的生长期和生长速率进行了研究。我们发现，在相同的近端和远端水平截肢的鳍鱼的生长周期和生长速度都不同，这表明直切后鳍条需要不同的时间段才能恢复到原来的长度。我们还表明，更多的近端截肢比更远端截肢需要更长的时间来重建原始的形态/尺寸。统计分析表明，两者的生长周期/速率都是由截断的长度(深度)决定的，而与背腹轴上的鳍线同一性无关。此外，我们认为结构/物理条件，如截肢处鳍线的宽度(残肢处的生态位)可能决定了生长周期/速率。
Fish have a high ability to regenerate fins, including the caudal fin. After caudal fin amputation, original bi-lobed morphology is reconstructed during its rapid regrowth. It is still controversial whether positional memory in the blastema cells regulates reconstruction of fin morphology as in amphibian limb regeneration, in which limb blastema cells located at the same proximal-distal level have the same positional identity. We investigated growth period and growth rate in zebrafish caudal fin regeneration. We found that both the growth period and growth rate differed for fin rays that were amputated at the same proximal-distal level, indicating that it takes different periods of time for fin rays to restore their original lengths after straight amputation. We also show that more proximal amputation takes longer period to reconstruct the original morphology/size than more distal amputation. Statistical analysis suggested that both the growth period/rate are determined by amputated length (depth) regardless of the fin ray identity along dorsal-ventral axis. In addition, we suggest the possibility that the structural/physical condition such as width of the fin ray at the amputation site (niche at the stump) may determine the growth period/rate.