Structure and genetic variation among populations of Euschistus heros from different geographic regions in Brazil
Structure and genetic variation among populations of Euschistus heros from different geographic regions in Brazil
复制标题
DOI：
10.1111/eea.12666
复制
发表时间：
2018-03
影响因子：
1.9
通讯作者：
P. Husch;D. G. Ferreira;N. Seraphim;N. Harvey;K. L. Silva-Brandão;S. Sofia;D. Sosa‐Gómez
中科院分区：
文献类型：
--
作者：
P. Husch;D. G. Ferreira;N. Seraphim;N. Harvey;K. L. Silva-Brandão;S. Sofia;D. Sosa‐Gómez
新热带区褐蝽（Euschistus heros（Fabricius），半翅目：蝽科）是巴西大豆的主要害虫。本研究利用微卫星标记技术，开发了一个新的微卫星位点，旨在为害虫抗药性管理提供相关信息。英雄并用于遗传多样性和种群结构的研究。使用来自巴西主要大豆产区（东北部，中西部和南部地区）的17个样品（n = 243个个体）的8个微卫星位点进行群体分析。这些微卫星位点提供了高的遗传多样性值在整个扩展的研究区域（他= 0.895，等位基因总数= 400）。新热带区褐蝽种群在样本中总体上显示出较低的遗传结构水平（总体Φ ST = 0.009）。东北地区的样品是一个例外，表现出显著的遗传分化（成对Φ ST = 0.031 - 0.063）。贝叶斯聚类分析证实了这些结果，没有显示人口细分，并表示相当程度的基因流。遗传差异与地理距离之间存在显著相关。在São Desidério的种群中发现了最低的迁移估计，根据遗传距离，这也是与其余种群最远的。这些群体间遗传分化较低的一些合理假设是大豆生产区的快速扩张，大豆是E.英雄，多化性，以及人为传播的可能影响。所有这些因素都可能导致种群密度高，分布广，可能有助于减少种群分化，增加遗传多样性。
The Neotropical brown stink bug, Euschistus heros (Fabricius) (Hemiptera: Pentatomidae), is a major Brazilian soybean pest. Aiming to provide relevant information to implement pest and insecticide resistance management, new microsatellite loci were developed for E. heros and used in a study of genetic diversity and population structure. The population analysis was performed using eight microsatellite loci from 17 samples (n = 243 individuals) collected in the major soybean‐producing regions in Brazil (northeastern, midwestern, and southern regions). These microsatellite loci provided high genetic diversity values on the whole extension for the studied region (He = 0.895; total number of alleles = 400). Neotropical brown stink bug populations in general displayed low genetic structure levels among the samples (overall ΦST = 0.009). An exception was the sample from the northeastern region, which showed a significant genetic differentiation (pairwise ΦST = 0.031–0.063). Bayesian cluster analysis confirmed these results, did not show population subdivision, and indicated considerable levels of gene flow. Significant correlations between genetic differences and geographic distance were obtained. The lowest estimate of migration was found in the population from São Desidério, which was also the most distant from the remaining populations based on genetic distance. Some plausible hypotheses for the low genetic differentiation among these populations are the fast expansion of soybean production areas, the main food source of E. heros, polyvoltinism, and possible influence of anthropogenic dispersal. All these factors could have led to high population densities, a wide distribution that may contribute to reduced population differentiation, and increased genetic diversity.