Creation of an electrokinetic characterization library for the detection and identification of biological cells
Creation of an electrokinetic characterization library for the detection and identification of biological cells
复制标题
DOI：
10.1007/s00216-020-02621-9
复制
发表时间：
2020-04-22
影响因子：
4.3
通讯作者：
Lapizco-Encinas, Blanca H.
中科院分区：
文献类型：
--
作者：
De Pena, Adriana Coll;Miller, Abbi;Lapizco-Encinas, Blanca H.
对耐药微生物的日益关注增加了对快速和便携式检测系统的需求。然而，用于微生物分析的传统方法可能是资源和时间密集型的。这一贡献提出了一种替代的方法，使用微尺度电动技术的微生物的表征。本研究的目的是开发和验证一个新的参数称为电动平衡条件为每个菌株，这将允许快速识别的研究的细菌和酵母细胞在电动（EK）微流控装置的库。为了创建文库，使用具有圆形柱的基于绝缘体的EK装置进行了六种感兴趣生物体的实验。这些微生物包括一种酵母菌株，酿酒酵母;一种沙门氏菌株，肠道沙门氏菌;两种来自同一属的菌种，蜡状芽孢杆菌和枯草芽孢杆菌;以及两种大肠杆菌菌株。然后用COMSOL Multiphysics（R）中的数学模型分析来自这些实验的结果，该数学模型产生每种不同菌株的动电平衡条件。最后，为了验证EK库的适用性，COMSOL模型用于估计具有椭圆形柱的装置中每种生物体所需的捕获条件，然后将这些值与实验获得的值进行比较。结果表明，该库可以用来估计陷阱电压的最大相对误差为12%。虽然所提出的电动技术仍然是一种新的方法，需要分析额外的微生物来扩大文库，但这一贡献进一步支持了微尺度电动技术作为一种快速和稳健表征微生物的技术的潜力。
The rising concern over drug-resistant microorganisms has increased the need for rapid and portable detection systems. However, the traditional methods for the analysis of microorganisms can be both resource and time intensive. This contribution presents an alternative approach for the characterization of microorganisms using a microscale electrokinetic technique. The present study aims to develop and validate a library with a novel parameter referred to as the electrokinetic equilibrium condition for each strain, which will allow for fast identification of the studied bacterial and yeast cells in electrokinetic (EK) microfluidic devices. To create the library, experiments with six organisms of interest were conducted using insulator-based EK devices with circle-shaped posts. The organisms included one yeast strain, Saccharomyces cerevisiae; one salmonella strain, Salmonella enterica; two species from the same genus, Bacillus cereus and Bacillus subtilis; and two Escherichia coli strains. The results from these experiments were then analyzed with a mathematical model in COMSOL Multiphysics(R), which yielded the electrokinetic equilibrium condition for each distinct strain. Lastly, to validate the applicability EK library, the COMSOL model was used to estimate the trapping conditions needed in a device with oval-shaped posts for each organism, and these values were then compared with experimentally obtained values. The results suggest the library can be used to estimate trapping voltages with a maximum relative error of 12%. While the proposed electrokinetic technique is still a novel approach and the analysis of additional microorganisms would be needed to expand the library, this contribution further supports the potential of microscale electrokinetics as a technique for the rapid and robust characterization of microbes.